@article{0e14d179791846f1a38535ab6ea81678,
title = "Elucidation of Gut Microbiota-Associated Lipids Using LC-MS/MS and 16S rRNA Sequence Analyses",
abstract = "Host-microbiota interactions create a unique metabolic milieu that modulates intestinal environments. Integration of 16S ribosomal RNA (rRNA) sequences and mass spectrometry (MS)-based lipidomics has a great potential to reveal the relationship between bacterial composition and the complex metabolic network in the gut. In this study, we conducted untargeted lipidomics followed by a feature-based molecular MS/MS spectral networking to characterize gut bacteria-dependent lipid subclasses in mice. An estimated 24.8% of lipid molecules in feces were microbiota-dependent, as judged by > 10-fold decrease in antibiotic-treated mice. Among these, there was a series of unique and microbiota-related lipid structures, including acyl alpha-hydroxyl fatty acid (AAHFA) that was newly identified in this study. Based on the integrated analysis of 985 lipid profiles and 16S rRNA sequence data providing 2,494 operational taxonomic units, we could successfully predict the bacterial species responsible for the biosynthesis of these unique lipids, including AAHFA.",
keywords = "Lipidomics, Microbiome, Omics",
author = "Shu Yasuda and Nobuyuki Okahashi and Hiroshi Tsugawa and Yusuke Ogata and Kazutaka Ikeda and Wataru Suda and Hiroyuki Arai and Masahira Hattori and Makoto Arita",
note = "Funding Information: This work was mainly supported by AMED LEAP under grant number JP18gm0010003 (for M.A.). The work was also supported partially by the JSPS Grant-in-Aid for Scientific Research on Innovative Areas “LipoQuality” ( 15H05897 , 15H05898 for M.A.) and JSPS KAKENHI ( 18H02432 , 18K19155 for H.T.). We thank our lab members, especially Akira Sen, Mie Honda, Aya Hori, Kanako Igarashi, Kaori Itaya, Yosuke Isobe, Masahiro Ueda, Kakou Ko, and Satoshi Morozumi, for their skillful technical supports and suggestions. We acknowledge Editage ( www.editage.jp ) for English language editing. Funding Information: This work was mainly supported by AMED LEAP under grant number JP18gm0010003 (for M.A.). The work was also supported partially by the JSPS Grant-in-Aid for Scientific Research on Innovative Areas ?LipoQuality? (15H05897, 15H05898 for M.A.) and JSPS KAKENHI (18H02432, 18K19155 for H.T.). We thank our lab members, especially Akira Sen, Mie Honda, Aya Hori, Kanako Igarashi, Kaori Itaya, Yosuke Isobe, Masahiro Ueda, Kakou Ko, and Satoshi Morozumi, for their skillful technical supports and suggestions. We acknowledge Editage (www.editage.jp) for English language editing. S.Y. and M.A. designed the study. S.Y. performed biological experiments. S.Y. and K.I. performed LC-MS/MS analyses, and Y.O. W.S. and M.H. performed 16S rRNA gene analyses. S.Y. N.O. and H.T. performed data analyses. S.Y. N.O. H.T. and M.A. wrote the manuscript. S.Y. N.O. H.T. H.A. and M.A. thoroughly discussed this project, and all authors helped improve the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = dec,
day = "18",
doi = "10.1016/j.isci.2020.101841",
language = "English",
volume = "23",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "12",
}