## Abstract

ABSTRACT. We developed a method to study the DNA synthetic cycles of Entamoeba histolytica and Entamoeba invadens by flow cytometry (FCM) based on a preparative procedure to reduce both high levels of natural fluorescence and non‐specific adsorption of fluorochromes. We modeled G_{1}, S, and G_{2} phases as a series of overlapping Gaussian curves. Both E. histolytica and E. invadens displayed G_{1}, S, and G_{2} proportions that are consistent with eukaryotic cell populations in exponential or stationary growth phase. Exponential phase E. histolytica populations contained a hypodiploid subset with a mass of about 20% less than the diploid value which we estimate by FCM to be 24 × 10^{‐14} g DNA/cell. Exponential phase E. invadens populations contained a hypodiploid subset with a mass of about 6% less than the diploid value which we estimate by FCM to be 30 × 10^{‐14} g DNA/cell.

Original language | English |
---|---|

Pages (from-to) | 610-616 |

Number of pages | 7 |

Journal | Journal of Eukaryotic Microbiology |

Volume | 42 |

Issue number | 5 |

DOIs | |

Publication status | Published - 1995 Sept |

## Keywords

- DNA synthetic cycle
- Entamoeba histolytica
- Entamoeba invadens
- flow cytometry
- mathematical modeling

## ASJC Scopus subject areas

- Microbiology