Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation

Miyuki Morozumi, Keiko Hasegawa, Reiko Kobayashi, Nagako Inoue, Satoshi Iwata, Haruo Kuroki, Naohisa Kawamura, Eiichi Nakayama, Takeshi Tajima, Kouichi Shimizu, Kimiko Ubukata

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

A total of 195 Mycoplasma pneumoniae strains were isolated from 2,462 clinical specimens collected between April 2002 and March 2004 from pédiatric outpatients with respiratory tract infections. Susceptibilities to six macrolide antibiotics (ML), telithromycin, minocycline, levofloxacin, and sitafloxacin were determined by the microdilution method using PPLO broth. A total of 183 M. pneumoniae isolates were susceptible to all agents and had excellent MIC90S in the following order: 0.00195 μg/ml for azithromycin and telithromycin, 0.0078 μg/ml for clarithromycin, 0.0156 μg/ml for erythromycin, 0.0625 μg/ml for sitafloxacin, 0.5 μg/ml for minocycline, and 1 μg/ml for levofloxacin. Notably, 12 ML-resistant M. pneumoniae strains were isolated from patients with pneumonia (10 strains) or acute bronchitis (2 strains). These strains showed resistance to ML with MICs of ≥1 μg/ml, except to rokitamycin. Transition mutations of A2063G or A2064G, which correspond to A2058 and A2059 in Escherichia coli, in domain V on the 23S rRNA gene in 11 ML-resistant strains were identified. By pulsed-field gel electrophoresis typing, these strains were classified into groups I and Vb, as described previously (A. Cousin-Allery, A. Charron, B. D. Barbeyrac, G. Fremy, J. S. Jensen, H. Renaudin, and C. Bebear, Epidemiol. Infect. 124:103-111, 2000). These findings suggest that excessive usage of MLs acts as a trigger to select mutations on the corresponding 23S rRNA gene with the resultant occurrence of ML-resistant M. pneumoniae. Monitoring ML susceptibilities for M. pneumoniae is necessary in the future.

Original languageEnglish
Pages (from-to)2302-2306
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume49
Issue number6
DOIs
Publication statusPublished - 2005 Jun
Externally publishedYes

Fingerprint

Mycoplasma pneumoniae
Macrolides
rRNA Genes
Anti-Bacterial Agents
Mutation
Levofloxacin
Minocycline
Azithromycin
Clarithromycin
Bronchitis
Mycoplasma
Pulsed Field Gel Electrophoresis
Erythromycin
Respiratory Tract Infections
Pneumonia
Outpatients
Escherichia coli

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation. / Morozumi, Miyuki; Hasegawa, Keiko; Kobayashi, Reiko; Inoue, Nagako; Iwata, Satoshi; Kuroki, Haruo; Kawamura, Naohisa; Nakayama, Eiichi; Tajima, Takeshi; Shimizu, Kouichi; Ubukata, Kimiko.

In: Antimicrobial Agents and Chemotherapy, Vol. 49, No. 6, 06.2005, p. 2302-2306.

Research output: Contribution to journalArticle

Morozumi, M, Hasegawa, K, Kobayashi, R, Inoue, N, Iwata, S, Kuroki, H, Kawamura, N, Nakayama, E, Tajima, T, Shimizu, K & Ubukata, K 2005, 'Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation', Antimicrobial Agents and Chemotherapy, vol. 49, no. 6, pp. 2302-2306. https://doi.org/10.1128/AAC.49.6.2302-2306.2005
Morozumi, Miyuki ; Hasegawa, Keiko ; Kobayashi, Reiko ; Inoue, Nagako ; Iwata, Satoshi ; Kuroki, Haruo ; Kawamura, Naohisa ; Nakayama, Eiichi ; Tajima, Takeshi ; Shimizu, Kouichi ; Ubukata, Kimiko. / Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation. In: Antimicrobial Agents and Chemotherapy. 2005 ; Vol. 49, No. 6. pp. 2302-2306.
@article{c5511fd1590643739330667d34fc4b57,
title = "Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation",
abstract = "A total of 195 Mycoplasma pneumoniae strains were isolated from 2,462 clinical specimens collected between April 2002 and March 2004 from p{\'e}diatric outpatients with respiratory tract infections. Susceptibilities to six macrolide antibiotics (ML), telithromycin, minocycline, levofloxacin, and sitafloxacin were determined by the microdilution method using PPLO broth. A total of 183 M. pneumoniae isolates were susceptible to all agents and had excellent MIC90S in the following order: 0.00195 μg/ml for azithromycin and telithromycin, 0.0078 μg/ml for clarithromycin, 0.0156 μg/ml for erythromycin, 0.0625 μg/ml for sitafloxacin, 0.5 μg/ml for minocycline, and 1 μg/ml for levofloxacin. Notably, 12 ML-resistant M. pneumoniae strains were isolated from patients with pneumonia (10 strains) or acute bronchitis (2 strains). These strains showed resistance to ML with MICs of ≥1 μg/ml, except to rokitamycin. Transition mutations of A2063G or A2064G, which correspond to A2058 and A2059 in Escherichia coli, in domain V on the 23S rRNA gene in 11 ML-resistant strains were identified. By pulsed-field gel electrophoresis typing, these strains were classified into groups I and Vb, as described previously (A. Cousin-Allery, A. Charron, B. D. Barbeyrac, G. Fremy, J. S. Jensen, H. Renaudin, and C. Bebear, Epidemiol. Infect. 124:103-111, 2000). These findings suggest that excessive usage of MLs acts as a trigger to select mutations on the corresponding 23S rRNA gene with the resultant occurrence of ML-resistant M. pneumoniae. Monitoring ML susceptibilities for M. pneumoniae is necessary in the future.",
author = "Miyuki Morozumi and Keiko Hasegawa and Reiko Kobayashi and Nagako Inoue and Satoshi Iwata and Haruo Kuroki and Naohisa Kawamura and Eiichi Nakayama and Takeshi Tajima and Kouichi Shimizu and Kimiko Ubukata",
year = "2005",
month = "6",
doi = "10.1128/AAC.49.6.2302-2306.2005",
language = "English",
volume = "49",
pages = "2302--2306",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation

AU - Morozumi, Miyuki

AU - Hasegawa, Keiko

AU - Kobayashi, Reiko

AU - Inoue, Nagako

AU - Iwata, Satoshi

AU - Kuroki, Haruo

AU - Kawamura, Naohisa

AU - Nakayama, Eiichi

AU - Tajima, Takeshi

AU - Shimizu, Kouichi

AU - Ubukata, Kimiko

PY - 2005/6

Y1 - 2005/6

N2 - A total of 195 Mycoplasma pneumoniae strains were isolated from 2,462 clinical specimens collected between April 2002 and March 2004 from pédiatric outpatients with respiratory tract infections. Susceptibilities to six macrolide antibiotics (ML), telithromycin, minocycline, levofloxacin, and sitafloxacin were determined by the microdilution method using PPLO broth. A total of 183 M. pneumoniae isolates were susceptible to all agents and had excellent MIC90S in the following order: 0.00195 μg/ml for azithromycin and telithromycin, 0.0078 μg/ml for clarithromycin, 0.0156 μg/ml for erythromycin, 0.0625 μg/ml for sitafloxacin, 0.5 μg/ml for minocycline, and 1 μg/ml for levofloxacin. Notably, 12 ML-resistant M. pneumoniae strains were isolated from patients with pneumonia (10 strains) or acute bronchitis (2 strains). These strains showed resistance to ML with MICs of ≥1 μg/ml, except to rokitamycin. Transition mutations of A2063G or A2064G, which correspond to A2058 and A2059 in Escherichia coli, in domain V on the 23S rRNA gene in 11 ML-resistant strains were identified. By pulsed-field gel electrophoresis typing, these strains were classified into groups I and Vb, as described previously (A. Cousin-Allery, A. Charron, B. D. Barbeyrac, G. Fremy, J. S. Jensen, H. Renaudin, and C. Bebear, Epidemiol. Infect. 124:103-111, 2000). These findings suggest that excessive usage of MLs acts as a trigger to select mutations on the corresponding 23S rRNA gene with the resultant occurrence of ML-resistant M. pneumoniae. Monitoring ML susceptibilities for M. pneumoniae is necessary in the future.

AB - A total of 195 Mycoplasma pneumoniae strains were isolated from 2,462 clinical specimens collected between April 2002 and March 2004 from pédiatric outpatients with respiratory tract infections. Susceptibilities to six macrolide antibiotics (ML), telithromycin, minocycline, levofloxacin, and sitafloxacin were determined by the microdilution method using PPLO broth. A total of 183 M. pneumoniae isolates were susceptible to all agents and had excellent MIC90S in the following order: 0.00195 μg/ml for azithromycin and telithromycin, 0.0078 μg/ml for clarithromycin, 0.0156 μg/ml for erythromycin, 0.0625 μg/ml for sitafloxacin, 0.5 μg/ml for minocycline, and 1 μg/ml for levofloxacin. Notably, 12 ML-resistant M. pneumoniae strains were isolated from patients with pneumonia (10 strains) or acute bronchitis (2 strains). These strains showed resistance to ML with MICs of ≥1 μg/ml, except to rokitamycin. Transition mutations of A2063G or A2064G, which correspond to A2058 and A2059 in Escherichia coli, in domain V on the 23S rRNA gene in 11 ML-resistant strains were identified. By pulsed-field gel electrophoresis typing, these strains were classified into groups I and Vb, as described previously (A. Cousin-Allery, A. Charron, B. D. Barbeyrac, G. Fremy, J. S. Jensen, H. Renaudin, and C. Bebear, Epidemiol. Infect. 124:103-111, 2000). These findings suggest that excessive usage of MLs acts as a trigger to select mutations on the corresponding 23S rRNA gene with the resultant occurrence of ML-resistant M. pneumoniae. Monitoring ML susceptibilities for M. pneumoniae is necessary in the future.

UR - http://www.scopus.com/inward/record.url?scp=21144458926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21144458926&partnerID=8YFLogxK

U2 - 10.1128/AAC.49.6.2302-2306.2005

DO - 10.1128/AAC.49.6.2302-2306.2005

M3 - Article

VL - 49

SP - 2302

EP - 2306

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 6

ER -