Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography

Shigeo Okubo, Fumiko Mashige, Mie Omori, Yoshiaki Hashimoto, Kazuhiko Nakahara, Hideko Kanazawa, Yoshikazu Matsushima

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Enantiomeric determination of L- and D-lactate in human cerebrospinal fluid (CSF) was achieved by HPLC on a chiral stationary phase with UV detection. Samples were submitted to a solid-phase extraction procedure using Oasis(TM) HLB Plus Extraction Cartridge and L- and D-lactate in the extract were separated by Shodex ORpac CRX-453 B column, a ligand exchange column for chiral separation, using a mobile phase containing copper (II) ion. L- and D-lactate were determined in 25 min. Intra-assay precision in CSF was 4.98% (mean 1.85 mmol/L) for L-lactate and 10.1% (mean 4.96 μmol/L) for D-lactate (n = 5). Detection limits were between 1.0 (L-lactate) and 1.5 (D-lactate) pmol. The mean values (n = 3) of analytical recovery for L- and D-lactate were 95% and 107%, respectively. The mean ± SD of concentrations of L- and D-lactate in CSF (n = 20) were 1.52 ± 0.54 mmol/L and 10.98 ± 5.15 μmol/L, respectively. Copyright (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish
Pages (from-to)474-477
Number of pages4
JournalBiomedical Chromatography
Volume14
Issue number7
DOIs
Publication statusPublished - 2000

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Cerebrospinal fluid
High performance liquid chromatography
Cerebrospinal Fluid
Lactic Acid
High Pressure Liquid Chromatography
Ligands
Solid Phase Extraction
Limit of Detection
Copper
Assays
Ion exchange
Ions
Recovery

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography. / Okubo, Shigeo; Mashige, Fumiko; Omori, Mie; Hashimoto, Yoshiaki; Nakahara, Kazuhiko; Kanazawa, Hideko; Matsushima, Yoshikazu.

In: Biomedical Chromatography, Vol. 14, No. 7, 2000, p. 474-477.

Research output: Contribution to journalArticle

Okubo, Shigeo ; Mashige, Fumiko ; Omori, Mie ; Hashimoto, Yoshiaki ; Nakahara, Kazuhiko ; Kanazawa, Hideko ; Matsushima, Yoshikazu. / Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography. In: Biomedical Chromatography. 2000 ; Vol. 14, No. 7. pp. 474-477.
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