Enantioselective oxidation of mandelic acid using a phenylmalonate metabolizing pathway of a soil bacterium Alcaligenesbronchisepticus KU 1201

Kenji Miyamoto, Hiromichi Ohta

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The metabolic pathway of phenylmalonic acid by Alcaligenesbronchisepticus KU1201, which catalyzes the asymmetric decarboxylation of α-aryl-α-methylmalonic acids to α-arylpropionic acids, is demonstrated. Enantioselective oxidation of mandelic acid was investigated using the metabolizing pathway in this strain. Incubation of (±)-mandelic acid with A.bronchisepticus afforded optically pure (R)-one accompanied by benzoylformic acid and benzoic acid. This enzymatic oxidation is also applicable to various substituted mandelic acids.

Original languageEnglish
Pages (from-to)363-366
Number of pages4
JournalBiotechnology Letters
Volume14
Issue number5
DOIs
Publication statusPublished - 1992 May

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Mandelic Acids
Bacteria
Soil
Methylmalonic Acid
Soils
Oxidation
Decarboxylation
Benzoic Acid
Acids
Metabolic Networks and Pathways
Benzoic acid
mandelic acid
phenylmalonic acid
phenylglyoxylic acid

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

Cite this

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abstract = "The metabolic pathway of phenylmalonic acid by Alcaligenesbronchisepticus KU1201, which catalyzes the asymmetric decarboxylation of α-aryl-α-methylmalonic acids to α-arylpropionic acids, is demonstrated. Enantioselective oxidation of mandelic acid was investigated using the metabolizing pathway in this strain. Incubation of (±)-mandelic acid with A.bronchisepticus afforded optically pure (R)-one accompanied by benzoylformic acid and benzoic acid. This enzymatic oxidation is also applicable to various substituted mandelic acids.",
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AU - Ohta, Hiromichi

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AB - The metabolic pathway of phenylmalonic acid by Alcaligenesbronchisepticus KU1201, which catalyzes the asymmetric decarboxylation of α-aryl-α-methylmalonic acids to α-arylpropionic acids, is demonstrated. Enantioselective oxidation of mandelic acid was investigated using the metabolizing pathway in this strain. Incubation of (±)-mandelic acid with A.bronchisepticus afforded optically pure (R)-one accompanied by benzoylformic acid and benzoic acid. This enzymatic oxidation is also applicable to various substituted mandelic acids.

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