TY - JOUR
T1 - Encapsulation of glucose oxidase (GOD) in polyelectrolyte complexes of chitosan-carrageenan
AU - Briones, Annabelle V.
AU - Sato, Toshinori
N1 - Funding Information:
Sincere thanks to Japan Society for the Promotion of Science (JSPS) for the financial support from the grant under the RONPAKU dissertation program and Keio University, as well as to the Industrial Technology Development Institute, and the Department of Science & Technology, Manila, Philippines.
PY - 2010/1
Y1 - 2010/1
N2 - The purpose of this study was to investigate the potential ability of carrageenan (κ-, ι-, λ-) and chitosan to form a controlled-release system for glucose oxidase (GOD). GOD was encapsulated in chitosan/carrageenan complexes at charge ratios (+/-) of 3 and 5 in mildly acidic solution. The encapsulation efficiency and activity of the loaded GOD were investigated. Among the different complexes prepared, chitosan/κ-carrageenan complex showed high encapsulation efficiencies of 79% and 62.5% at charge ratios of 3 and 5, respectively. The order of encapsulation efficiency decreases toward chitosan/λ-carrageenan complex (κ > ι > λ). After treatment with chitosanase and pepsin solutions, the activity of encapsulated glucose oxidase (GOD) was preserved for all complexes. The chitosan/κ-carrageenan complex was able to preserve 80.2% of GOD activity in pH 1.2 solution, 73.3% in chitosanase solution and 66.4% in pepsin solution. Controlled release of GOD was observed when the complexes were treated with different physiological and enzyme solutions; the complex of chitosan/κ-carrageenan had the lowest release rate of GOD. The simple preparation of chitosan/carrageenan complexes and their ability to protect protein integrity under acidic conditions make them a promising drug delivery system for the oral administration of peptides and proteins.
AB - The purpose of this study was to investigate the potential ability of carrageenan (κ-, ι-, λ-) and chitosan to form a controlled-release system for glucose oxidase (GOD). GOD was encapsulated in chitosan/carrageenan complexes at charge ratios (+/-) of 3 and 5 in mildly acidic solution. The encapsulation efficiency and activity of the loaded GOD were investigated. Among the different complexes prepared, chitosan/κ-carrageenan complex showed high encapsulation efficiencies of 79% and 62.5% at charge ratios of 3 and 5, respectively. The order of encapsulation efficiency decreases toward chitosan/λ-carrageenan complex (κ > ι > λ). After treatment with chitosanase and pepsin solutions, the activity of encapsulated glucose oxidase (GOD) was preserved for all complexes. The chitosan/κ-carrageenan complex was able to preserve 80.2% of GOD activity in pH 1.2 solution, 73.3% in chitosanase solution and 66.4% in pepsin solution. Controlled release of GOD was observed when the complexes were treated with different physiological and enzyme solutions; the complex of chitosan/κ-carrageenan had the lowest release rate of GOD. The simple preparation of chitosan/carrageenan complexes and their ability to protect protein integrity under acidic conditions make them a promising drug delivery system for the oral administration of peptides and proteins.
KW - Carrageenan
KW - Chitosan
KW - Controlled-release system
KW - Micro-encapsulation
KW - Protein delivery
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U2 - 10.1016/j.reactfunctpolym.2009.09.009
DO - 10.1016/j.reactfunctpolym.2009.09.009
M3 - Article
AN - SCOPUS:70749148577
VL - 70
SP - 19
EP - 27
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
SN - 1381-5148
IS - 1
ER -