Endogenous glutamatergic synaptic activity elicits acetylcholine release from rat cultured septal cells

Takeshi Suzuki; Takeshi Matsugi; Ryoichi Takagi; Koichiro Kawashima

doi:10.1016/S0168-0102(03)00221-9

Endogenous glutamatergic synaptic activity elicits acetylcholine release from rat cultured septal cells

Takeshi Suzuki, Takeshi Matsugi, Ryoichi Takagi, Koichiro Kawashima

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We tested the characteristics of acetylcholine (ACh) release from cultured rat septal cells. The spontaneous release was inhibited by treatment with tetrodotoxin (TTX) and omega-conotoxin (GVIA), indicating that the release was elicited by synaptic activity. The release was also inhibited by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor blocker, in both the absence and presence of nerve growth factor (NGF), suggesting that endogenously released glutamate produced the ACh release by stimulating AMPA receptors. This is the first report of detection of the release of ACh by endogenous spontaneous synaptic activity conducted by glutamate AMPA receptor activation in cultured septal cells. This in vitro experimental system is useful for the study of cholinergic functions.

Original language	English
Pages (from-to)	341-347
Number of pages	7
Journal	Neuroscience Research
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/S0168-0102(03)00221-9
Publication status	Published - 2003 Nov 1

Keywords

AMPA
CNQX
Calcium
Choline acetyltransferase
Nerve growth factor
Septum
Tetrodotoxin
Vesamicol

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0168-0102(03)00221-9

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title = "Endogenous glutamatergic synaptic activity elicits acetylcholine release from rat cultured septal cells",

abstract = "We tested the characteristics of acetylcholine (ACh) release from cultured rat septal cells. The spontaneous release was inhibited by treatment with tetrodotoxin (TTX) and omega-conotoxin (GVIA), indicating that the release was elicited by synaptic activity. The release was also inhibited by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor blocker, in both the absence and presence of nerve growth factor (NGF), suggesting that endogenously released glutamate produced the ACh release by stimulating AMPA receptors. This is the first report of detection of the release of ACh by endogenous spontaneous synaptic activity conducted by glutamate AMPA receptor activation in cultured septal cells. This in vitro experimental system is useful for the study of cholinergic functions.",

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AU - Matsugi, Takeshi

AU - Takagi, Ryoichi

AU - Kawashima, Koichiro

PY - 2003/11/1

Y1 - 2003/11/1

N2 - We tested the characteristics of acetylcholine (ACh) release from cultured rat septal cells. The spontaneous release was inhibited by treatment with tetrodotoxin (TTX) and omega-conotoxin (GVIA), indicating that the release was elicited by synaptic activity. The release was also inhibited by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor blocker, in both the absence and presence of nerve growth factor (NGF), suggesting that endogenously released glutamate produced the ACh release by stimulating AMPA receptors. This is the first report of detection of the release of ACh by endogenous spontaneous synaptic activity conducted by glutamate AMPA receptor activation in cultured septal cells. This in vitro experimental system is useful for the study of cholinergic functions.

AB - We tested the characteristics of acetylcholine (ACh) release from cultured rat septal cells. The spontaneous release was inhibited by treatment with tetrodotoxin (TTX) and omega-conotoxin (GVIA), indicating that the release was elicited by synaptic activity. The release was also inhibited by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor blocker, in both the absence and presence of nerve growth factor (NGF), suggesting that endogenously released glutamate produced the ACh release by stimulating AMPA receptors. This is the first report of detection of the release of ACh by endogenous spontaneous synaptic activity conducted by glutamate AMPA receptor activation in cultured septal cells. This in vitro experimental system is useful for the study of cholinergic functions.

KW - AMPA

KW - CNQX

KW - Calcium

KW - Choline acetyltransferase

KW - Nerve growth factor

KW - Septum

KW - Tetrodotoxin

KW - Vesamicol

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Endogenous glutamatergic synaptic activity elicits acetylcholine release from rat cultured septal cells

Abstract

Keywords

ASJC Scopus subject areas

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