Endogenous glutamatergic synaptic activity elicits acetylcholine release from rat cultured septal cells

Takeshi Suzuki, Takeshi Matsugi, Ryoichi Takagi, Koichiro Kawashima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We tested the characteristics of acetylcholine (ACh) release from cultured rat septal cells. The spontaneous release was inhibited by treatment with tetrodotoxin (TTX) and omega-conotoxin (GVIA), indicating that the release was elicited by synaptic activity. The release was also inhibited by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor blocker, in both the absence and presence of nerve growth factor (NGF), suggesting that endogenously released glutamate produced the ACh release by stimulating AMPA receptors. This is the first report of detection of the release of ACh by endogenous spontaneous synaptic activity conducted by glutamate AMPA receptor activation in cultured septal cells. This in vitro experimental system is useful for the study of cholinergic functions.

Original languageEnglish
Pages (from-to)341-347
Number of pages7
JournalNeuroscience Research
Volume47
Issue number3
DOIs
Publication statusPublished - 2003 Nov 1

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Keywords

  • AMPA
  • CNQX
  • Calcium
  • Choline acetyltransferase
  • Nerve growth factor
  • Septum
  • Tetrodotoxin
  • Vesamicol

ASJC Scopus subject areas

  • Neuroscience(all)

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