Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion

Masaya Ueno, Katsuhide Igarashi, Naoki Kimura, Keisuke Okita, Makiko Takizawa, Ikuo Nobuhisa, Tetsuo Kojima, Toshio Kitamura, Ulrike Samulowitz, Dietmar Vestweber, Taizo Shimomura, Toshio Suda, Kinichi Nakashima, Tetsuya Taga

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.

Original languageEnglish
Pages (from-to)501-506
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number2
DOIs
Publication statusPublished - 2001 Sep 21

Fingerprint

Sialomucins
Cell adhesion
Cell Adhesion
Aorta
Endothelial cells
Endothelial Cells
Hematopoiesis
Hemangioblasts
Sialoglycoproteins
Cell Aggregation
Osteopontin
Protein Sorting Signals
Gene Library
Endothelium
Cultured Cells
Adhesion
Agglomeration
Embryonic Structures
Clone Cells
Immunohistochemistry

Keywords

  • Aorta-gonad-mesonephros
  • Cell adhesion
  • Cell aggregation
  • Endomucin
  • Endothelial cells
  • Hemangioblasts
  • Hematopoiesis
  • Hematopoietic stem cells
  • Sialomucin
  • Signal sequence trap

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ueno, M., Igarashi, K., Kimura, N., Okita, K., Takizawa, M., Nobuhisa, I., ... Taga, T. (2001). Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. Biochemical and Biophysical Research Communications, 287(2), 501-506. https://doi.org/10.1006/bbrc.2001.5587

Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. / Ueno, Masaya; Igarashi, Katsuhide; Kimura, Naoki; Okita, Keisuke; Takizawa, Makiko; Nobuhisa, Ikuo; Kojima, Tetsuo; Kitamura, Toshio; Samulowitz, Ulrike; Vestweber, Dietmar; Shimomura, Taizo; Suda, Toshio; Nakashima, Kinichi; Taga, Tetsuya.

In: Biochemical and Biophysical Research Communications, Vol. 287, No. 2, 21.09.2001, p. 501-506.

Research output: Contribution to journalArticle

Ueno, M, Igarashi, K, Kimura, N, Okita, K, Takizawa, M, Nobuhisa, I, Kojima, T, Kitamura, T, Samulowitz, U, Vestweber, D, Shimomura, T, Suda, T, Nakashima, K & Taga, T 2001, 'Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion', Biochemical and Biophysical Research Communications, vol. 287, no. 2, pp. 501-506. https://doi.org/10.1006/bbrc.2001.5587
Ueno, Masaya ; Igarashi, Katsuhide ; Kimura, Naoki ; Okita, Keisuke ; Takizawa, Makiko ; Nobuhisa, Ikuo ; Kojima, Tetsuo ; Kitamura, Toshio ; Samulowitz, Ulrike ; Vestweber, Dietmar ; Shimomura, Taizo ; Suda, Toshio ; Nakashima, Kinichi ; Taga, Tetsuya. / Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 287, No. 2. pp. 501-506.
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