Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion

Masaya Ueno; Katsuhide Igarashi; Naoki Kimura; Keisuke Okita; Makiko Takizawa; Ikuo Nobuhisa; Tetsuo Kojima; Toshio Kitamura; Ulrike Samulowitz; Dietmar Vestweber; Taizo Shimomura; Toshio Suda; Kinichi Nakashima; Tetsuya Taga

doi:10.1006/bbrc.2001.5587

Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion

Masaya Ueno, Katsuhide Igarashi, Naoki Kimura, Keisuke Okita, Makiko Takizawa, Ikuo Nobuhisa, Tetsuo Kojima, Toshio Kitamura, Ulrike Samulowitz, Dietmar Vestweber, Taizo Shimomura, Toshio Suda, Kinichi Nakashima, Tetsuya Taga

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.

Original language	English
Pages (from-to)	501-506
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	287
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.2001.5587
Publication status	Published - 2001 Sep 21
Externally published	Yes

Keywords

Aorta-gonad-mesonephros
Cell adhesion
Cell aggregation
Endomucin
Endothelial cells
Hemangioblasts
Hematopoiesis
Hematopoietic stem cells
Sialomucin
Signal sequence trap

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.2001.5587

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Ueno, M., Igarashi, K., Kimura, N., Okita, K., Takizawa, M., Nobuhisa, I., Kojima, T., Kitamura, T., Samulowitz, U., Vestweber, D., Shimomura, T., Suda, T., Nakashima, K., & Taga, T. (2001). Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. Biochemical and Biophysical Research Communications, 287(2), 501-506. https://doi.org/10.1006/bbrc.2001.5587

Ueno, M, Igarashi, K, Kimura, N, Okita, K, Takizawa, M, Nobuhisa, I, Kojima, T, Kitamura, T, Samulowitz, U, Vestweber, D, Shimomura, T, Suda, T, Nakashima, K & Taga, T 2001, 'Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion', Biochemical and Biophysical Research Communications, vol. 287, no. 2, pp. 501-506. https://doi.org/10.1006/bbrc.2001.5587

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title = "Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion",

abstract = "Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.",

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author = "Masaya Ueno and Katsuhide Igarashi and Naoki Kimura and Keisuke Okita and Makiko Takizawa and Ikuo Nobuhisa and Tetsuo Kojima and Toshio Kitamura and Ulrike Samulowitz and Dietmar Vestweber and Taizo Shimomura and Toshio Suda and Kinichi Nakashima and Tetsuya Taga",

note = "Funding Information: We are very much grateful to Ms. Yuki Noguchi for her excellent secretarial assistance. We also thank Ms. Kaori Kaneko for her technical help. This work was supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Takeda Science Foundation, and Human Frontier Science Program.",

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doi = "10.1006/bbrc.2001.5587",

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AU - Ueno, Masaya

AU - Igarashi, Katsuhide

AU - Kimura, Naoki

AU - Okita, Keisuke

AU - Takizawa, Makiko

AU - Nobuhisa, Ikuo

AU - Kojima, Tetsuo

AU - Kitamura, Toshio

AU - Samulowitz, Ulrike

AU - Vestweber, Dietmar

AU - Shimomura, Taizo

AU - Suda, Toshio

AU - Nakashima, Kinichi

AU - Taga, Tetsuya

N1 - Funding Information: We are very much grateful to Ms. Yuki Noguchi for her excellent secretarial assistance. We also thank Ms. Kaori Kaneko for her technical help. This work was supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Takeda Science Foundation, and Human Frontier Science Program.

PY - 2001/9/21

Y1 - 2001/9/21

N2 - Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.

AB - Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.

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KW - Endomucin

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KW - Hemangioblasts

KW - Hematopoiesis

KW - Hematopoietic stem cells

KW - Sialomucin

KW - Signal sequence trap

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Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion

Abstract

Keywords

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