Abstract
Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.
| Original language | English |
|---|---|
| Pages (from-to) | 501-506 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 287 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2001 Sep 21 |
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Keywords
- Aorta-gonad-mesonephros
- Cell adhesion
- Cell aggregation
- Endomucin
- Endothelial cells
- Hemangioblasts
- Hematopoiesis
- Hematopoietic stem cells
- Sialomucin
- Signal sequence trap
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. / Ueno, Masaya; Igarashi, Katsuhide; Kimura, Naoki; Okita, Keisuke; Takizawa, Makiko; Nobuhisa, Ikuo; Kojima, Tetsuo; Kitamura, Toshio; Samulowitz, Ulrike; Vestweber, Dietmar; Shimomura, Taizo; Suda, Toshio; Nakashima, Kinichi; Taga, Tetsuya.
In: Biochemical and Biophysical Research Communications, Vol. 287, No. 2, 21.09.2001, p. 501-506.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion
AU - Ueno, Masaya
AU - Igarashi, Katsuhide
AU - Kimura, Naoki
AU - Okita, Keisuke
AU - Takizawa, Makiko
AU - Nobuhisa, Ikuo
AU - Kojima, Tetsuo
AU - Kitamura, Toshio
AU - Samulowitz, Ulrike
AU - Vestweber, Dietmar
AU - Shimomura, Taizo
AU - Suda, Toshio
AU - Nakashima, Kinichi
AU - Taga, Tetsuya
PY - 2001/9/21
Y1 - 2001/9/21
N2 - Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.
AB - Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.
KW - Aorta-gonad-mesonephros
KW - Cell adhesion
KW - Cell aggregation
KW - Endomucin
KW - Endothelial cells
KW - Hemangioblasts
KW - Hematopoiesis
KW - Hematopoietic stem cells
KW - Sialomucin
KW - Signal sequence trap
UR - http://www.scopus.com/inward/record.url?scp=0035929358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035929358&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2001.5587
DO - 10.1006/bbrc.2001.5587
M3 - Article
C2 - 11554756
AN - SCOPUS:0035929358
VL - 287
SP - 501
EP - 506
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -