Abstract
Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.
Original language | English |
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Pages (from-to) | 501-506 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 287 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2001 Sep 21 |
Externally published | Yes |
Keywords
- Aorta-gonad-mesonephros
- Cell adhesion
- Cell aggregation
- Endomucin
- Endothelial cells
- Hemangioblasts
- Hematopoiesis
- Hematopoietic stem cells
- Sialomucin
- Signal sequence trap
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology