Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion

Masaya Ueno; Katsuhide Igarashi; Naoki Kimura; Keisuke Okita; Makiko Takizawa; Ikuo Nobuhisa; Tetsuo Kojima; Toshio Kitamura; Ulrike Samulowitz; Dietmar Vestweber; Taizo Shimomura; Toshio Suda; Kinichi Nakashima; Tetsuya Taga

doi:10.1006/bbrc.2001.5587

Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion

Masaya Ueno, Katsuhide Igarashi, Naoki Kimura, Keisuke Okita, Makiko Takizawa, Ikuo Nobuhisa, Tetsuo Kojima, Toshio Kitamura, Ulrike Samulowitz, Dietmar Vestweber, Taizo Shimomura, Toshio Suda, Kinichi Nakashima, Tetsuya Taga

The Sakaguchi Laboratory of Developmental Biology Department of Cell Differentiation

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.

Original language	English
Pages (from-to)	501-506
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	287
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.2001.5587
Publication status	Published - 2001 Sep 21

Keywords

Aorta-gonad-mesonephros
Cell adhesion
Cell aggregation
Endomucin
Endothelial cells
Hemangioblasts
Hematopoiesis
Hematopoietic stem cells
Sialomucin
Signal sequence trap

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.2001.5587

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Ueno, M., Igarashi, K., Kimura, N., Okita, K., Takizawa, M., Nobuhisa, I., Kojima, T., Kitamura, T., Samulowitz, U., Vestweber, D., Shimomura, T., Suda, T., Nakashima, K., & Taga, T. (2001). Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. Biochemical and Biophysical Research Communications, 287(2), 501-506. https://doi.org/10.1006/bbrc.2001.5587

Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. / Ueno, Masaya; Igarashi, Katsuhide; Kimura, Naoki; Okita, Keisuke; Takizawa, Makiko; Nobuhisa, Ikuo; Kojima, Tetsuo; Kitamura, Toshio; Samulowitz, Ulrike; Vestweber, Dietmar; Shimomura, Taizo; Suda, Toshio; Nakashima, Kinichi; Taga, Tetsuya.

In: Biochemical and Biophysical Research Communications, Vol. 287, No. 2, 21.09.2001, p. 501-506.

Research output: Contribution to journal › Article › peer-review

Ueno, M, Igarashi, K, Kimura, N, Okita, K, Takizawa, M, Nobuhisa, I, Kojima, T, Kitamura, T, Samulowitz, U, Vestweber, D, Shimomura, T, Suda, T, Nakashima, K & Taga, T 2001, 'Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion', Biochemical and Biophysical Research Communications, vol. 287, no. 2, pp. 501-506. https://doi.org/10.1006/bbrc.2001.5587

Ueno, Masaya ; Igarashi, Katsuhide ; Kimura, Naoki ; Okita, Keisuke ; Takizawa, Makiko ; Nobuhisa, Ikuo ; Kojima, Tetsuo ; Kitamura, Toshio ; Samulowitz, Ulrike ; Vestweber, Dietmar ; Shimomura, Taizo ; Suda, Toshio ; Nakashima, Kinichi ; Taga, Tetsuya. / Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 287, No. 2. pp. 501-506.

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abstract = "Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.",

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AU - Kojima, Tetsuo

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AU - Suda, Toshio

AU - Nakashima, Kinichi

AU - Taga, Tetsuya

N1 - Funding Information: We are very much grateful to Ms. Yuki Noguchi for her excellent secretarial assistance. We also thank Ms. Kaori Kaneko for her technical help. This work was supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Takeda Science Foundation, and Human Frontier Science Program.

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N2 - Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.

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Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion

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