Endothelial constitutive nitric oxide synthase protein and mRNA increased in rabbit atherosclerotic aorta despite impaired endothelium-dependent vascular relaxation

Kenji Kanazawa, Seinosuke Kawashima, Shuji Mikami, Yoichi Miwa, Ken Ichi Hirata, Masakuni Suematsu, Yoshitake Hayashi, Hiroshi Itoh, Mitsuhiro Yokoyama

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90 Citations (Scopus)

Abstract

Atherosclerotic arteries are well known to exhibit impaired endothelium-dependent relaxation (EDR), but the exact mechanism of this impairment remains unclear. Recently, endothelial constitutive nitric oxide synthase (ECNOS), which generates nitric oxide and mediates EDR, was cloned, and ECNOS mRNA expression was reported to be modified by various cytokines, lipoproteins, and shear stress. To investigate the expression of ECNOS mRNA and protein in atherosclerotic arteries with impaired EDR, thoracic aortas isolated from Watanabe heritable hyperlipidemic (WHHL) rabbits were examined by using in situ hybridization and immunohistochemistry. Compared with thoracic aortas from Japanese White rabbits, WaHL aortas exhibited significantly impaired EDRs, although both in situ hybridization and immunohistochemistry exhibited enhanced expression of ECNOS mRNA and protein in WHHL aortas. There was no significant relationship between expression of ECNOS mRNA and protein of endothelial cells and age of the examined WHHL aortas. These data suggest that the mechanism of impaired EDR in atherosclerotic arteries is not due to the decrease in ECNOS mRNA and protein.

Original languageEnglish
Pages (from-to)1949-1956
Number of pages8
JournalAmerican Journal of Pathology
Volume148
Issue number6
Publication statusPublished - 1996 Jun 1
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Kanazawa, K., Kawashima, S., Mikami, S., Miwa, Y., Hirata, K. I., Suematsu, M., Hayashi, Y., Itoh, H., & Yokoyama, M. (1996). Endothelial constitutive nitric oxide synthase protein and mRNA increased in rabbit atherosclerotic aorta despite impaired endothelium-dependent vascular relaxation. American Journal of Pathology, 148(6), 1949-1956.