Endothelial growth factor receptors in human fetal heart

Taina A. Partanen, Taija Makinen, Johanna Arola, Toshio Suda, Herbert A. Weich, Kari Alitalo

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background - Endothelial receptor tyrosine kinases include 3 members of the vascular endothelial growth factor receptor (VEGFR) family and 2 members of the angiopoietin receptor (Tie) family. In addition, the VEGF165 isoform binds to neuropilin-1 (NP-1), a receptor for collapsins/semaphorins. The importance of these receptors for vasculogenesis and angiogenesis has been shown in gene-targeted mice, but so far, little is known about their exact expression patterns in the human vasculature. Methods and Results - Frozen sections of human fetal heart were stained immunohistochemically with receptor-specific monoclonal (VEGFR, Tie) or polyclonal (NP-1) antibodies. The following patterns were observed. The endocardium was positive for VEGFR- 1, VEGFR-2, NP-1, Tie-1, and Tie-2 but negative for VEGFR-3. The coronary vessels were positive for Tie-1, Tie-2, VEGFR-1, and NP-1 and negative for VEGFR-2 and VEGFR-3. Myocardial capillaries and epicardial blood vessels stained for VEGFR-1, VEGFR-2, NP-1, and Tie-1; myocardial capillaries and epicardial veins weakly for Tie-2; and epicardial lymphatic vessels for VEGFR-2 and VEGFR-3, weakly for Tie-1 and Tie-2, but not for VEGFR-1 or NP- 1. Conclusions - The results demonstrate differential expression of the endothelial growth factor receptors in distinct types of vessels in the human heart. This information is useful for the understanding of their roles in physiological and pathological processes and for their diagnostic and therapeutic application in cardiovascular medicine.

Original languageEnglish
Pages (from-to)583-586
Number of pages4
JournalCirculation
Volume100
Issue number6
Publication statusPublished - 1999 Aug 10

Fingerprint

Neuropilin-1
Fetal Heart
Vascular Endothelial Growth Factor Receptor-2
Vascular Endothelial Growth Factor Receptor
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-3
Semaphorin-3A
Angiopoietins
Semaphorins
Physiological Phenomena
Endocardium
Lymphatic Vessels
Frozen Sections
Receptor Protein-Tyrosine Kinases
Pathologic Processes
Blood Vessels
Veins
Coronary Vessels
Protein Isoforms
Medicine

Keywords

  • Angiogenesis
  • Endocardium
  • Endothelium
  • Growth substances
  • Myocardium

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Partanen, T. A., Makinen, T., Arola, J., Suda, T., Weich, H. A., & Alitalo, K. (1999). Endothelial growth factor receptors in human fetal heart. Circulation, 100(6), 583-586.

Endothelial growth factor receptors in human fetal heart. / Partanen, Taina A.; Makinen, Taija; Arola, Johanna; Suda, Toshio; Weich, Herbert A.; Alitalo, Kari.

In: Circulation, Vol. 100, No. 6, 10.08.1999, p. 583-586.

Research output: Contribution to journalArticle

Partanen, TA, Makinen, T, Arola, J, Suda, T, Weich, HA & Alitalo, K 1999, 'Endothelial growth factor receptors in human fetal heart', Circulation, vol. 100, no. 6, pp. 583-586.
Partanen TA, Makinen T, Arola J, Suda T, Weich HA, Alitalo K. Endothelial growth factor receptors in human fetal heart. Circulation. 1999 Aug 10;100(6):583-586.
Partanen, Taina A. ; Makinen, Taija ; Arola, Johanna ; Suda, Toshio ; Weich, Herbert A. ; Alitalo, Kari. / Endothelial growth factor receptors in human fetal heart. In: Circulation. 1999 ; Vol. 100, No. 6. pp. 583-586.
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AU - Arola, Johanna

AU - Suda, Toshio

AU - Weich, Herbert A.

AU - Alitalo, Kari

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AB - Background - Endothelial receptor tyrosine kinases include 3 members of the vascular endothelial growth factor receptor (VEGFR) family and 2 members of the angiopoietin receptor (Tie) family. In addition, the VEGF165 isoform binds to neuropilin-1 (NP-1), a receptor for collapsins/semaphorins. The importance of these receptors for vasculogenesis and angiogenesis has been shown in gene-targeted mice, but so far, little is known about their exact expression patterns in the human vasculature. Methods and Results - Frozen sections of human fetal heart were stained immunohistochemically with receptor-specific monoclonal (VEGFR, Tie) or polyclonal (NP-1) antibodies. The following patterns were observed. The endocardium was positive for VEGFR- 1, VEGFR-2, NP-1, Tie-1, and Tie-2 but negative for VEGFR-3. The coronary vessels were positive for Tie-1, Tie-2, VEGFR-1, and NP-1 and negative for VEGFR-2 and VEGFR-3. Myocardial capillaries and epicardial blood vessels stained for VEGFR-1, VEGFR-2, NP-1, and Tie-1; myocardial capillaries and epicardial veins weakly for Tie-2; and epicardial lymphatic vessels for VEGFR-2 and VEGFR-3, weakly for Tie-1 and Tie-2, but not for VEGFR-1 or NP- 1. Conclusions - The results demonstrate differential expression of the endothelial growth factor receptors in distinct types of vessels in the human heart. This information is useful for the understanding of their roles in physiological and pathological processes and for their diagnostic and therapeutic application in cardiovascular medicine.

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