Endothelin-3 controls growth of colonic epithelial cells by mediating epithelial-mesenchymal interaction

Hiroshi Fukamachi, Takanori Narita, Naohisa Yahagi, Hiroyuki Takeda, Masao Ichinose

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

It has been repeatedly reported that endothelin-3 (ET-3) is expressed by gastrointestinal mesenchymes, and that paracrine signaling between ET-3 and its receptor plays an essential role in controlling differentiation of the enteric nervous system in the gut, especially in the colon. However it remains to be solved whether ET-3 plays a role in regulating the growth of gastrointestinal epithelial cells. We have previously reported culture systems for forestomach, glandular stomach and duodenal epithelial cells, but a system for colonic epithelial cells has not been established. In the present study, we examined optimal culture conditions for colonic epithelial cells, and examined whether ET-3 affects the growth of gastrointestinal epithelial cells, with special reference to colonic cells. We found that ET-3 dose-dependently and region-specifically stimulated their growth in primary culture: colonic epithelial cells were most responsive, followed by duodenal and glandular stomach epithelial cells. Reverse transcription-polymerase chain reaction analysis showed that ET-3 and a receptor for ET-3 were expressed by both colonic mesenchymes and epithelia, but the levels were much higher in mesenchymes than in epithelia. These results suggest that ET-3 plays an important role in the growth control of colonic epithelial cells, possibly by mediating epithelial-mesenchymal interactions.

Original languageEnglish
Pages (from-to)573-580
Number of pages8
JournalDevelopment Growth and Differentiation
Volume47
Issue number8
DOIs
Publication statusPublished - 2005 Oct
Externally publishedYes

Fingerprint

Endothelin-3
Epithelial Cells
Growth
Mesoderm
Stomach
Epithelium
Paracrine Communication
Enteric Nervous System
Reverse Transcription
Colon
Polymerase Chain Reaction

Keywords

  • Colon
  • Duodenum
  • Endothelin-3
  • Epithelial-mesenchymal interaction
  • Primary culture

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Endothelin-3 controls growth of colonic epithelial cells by mediating epithelial-mesenchymal interaction. / Fukamachi, Hiroshi; Narita, Takanori; Yahagi, Naohisa; Takeda, Hiroyuki; Ichinose, Masao.

In: Development Growth and Differentiation, Vol. 47, No. 8, 10.2005, p. 573-580.

Research output: Contribution to journalArticle

Fukamachi, Hiroshi ; Narita, Takanori ; Yahagi, Naohisa ; Takeda, Hiroyuki ; Ichinose, Masao. / Endothelin-3 controls growth of colonic epithelial cells by mediating epithelial-mesenchymal interaction. In: Development Growth and Differentiation. 2005 ; Vol. 47, No. 8. pp. 573-580.
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