Endothelin in human plasma and culture medium of aortic endothelial cells-Detection and characterization with radioimmunoassay using monoclonal antibody

Yoshihiko Saito, Kazuwa Nakao, Hiroshi Itoh, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Gotaro Shirakami, Shin ichi Suga, Michihisa Jougasaki, Shoko Morichika, Hiroo Imura

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

We have developed monoclonal (KY-ET-1-I) and polyclonal (ET-F5) antibodies against endothelin-1 (ET-1) and established sensitive radioimmunoassays (RIAs) with different specificities. The RIA with KY-ET-1-I detected ET-1, ET-2 and ET-3, while the RIA with ET-F5 recognized ET-3 very weakly. Using these RIAs, we have investigated the concentration and molecular forms of ET-1-like immunoreactivity (-LI) in culture medium of bovine aortic endothelial cells and human plasma. Culture medium of endothelial cells contained two major components compatible with big ET and ET-1. ET-1-LI was also detected in human plasma. ET-1-LI in human plasma consisted of apparent two components, the small molecular form emerging at the position of ET-1 and the large form with the peak eluting at the preceding fraction of the elution position of big ET. The concentration of the small form of ET in human plasma was about 5 pg/ml.

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number1
DOIs
Publication statusPublished - 1989 May 30
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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