Endothelins promote egg albumin-induced intestinal anaphylaxis in rats

T. Shigematsu, S. Miura, M. Hirokawa, R. Hokari, H. Higuchi, Y. Tsuzuki, H. Kimura, R. C. Nakatsumi, H. Serizawa, Hidetsugu Saito, H. Ishii

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background and Aims: The basic mechanisms of food allergies are still unknown. The aims of this study were to investigate whether endothelins (ETs) in the intestinal mucosa are involved in the pathogenesis of intestinal anaphylaxis. Methods: Sprague-Dawley rats were sensitized to chicken egg albumin (EA) by intraperitoneal injection. Fourteen days after sensitization, EA was administered in the jejunal segments to induce intestinal anaphylaxis. Net water outflux and histamine release into loops and serum concentrations of rat mast cell protease II (RMCP-II) were determined. ET-1 and ET-3 concentrations in the jejunal mucosa were determined, and expression of the corresponding messenger RNAs was examined by competitive polymerase chain reaction. Results: In sensitized animals, challenge with intraluminal antigen caused a significant increase in net water outflux and histamine release together with an elevation of serum Rmcp-ii concentrations. Mucosal concentrations of ET-1 and ET-3 and expression of their messenger Rnas were significantly increased in sensitized animals after EA challenge. Treatment with an ET(A)-receptor antagonist, but not an ET(B)-receptor antagonist, attenuated the increase in net water outflux, histamine release, and serum Rmcp-ii concentrations in rats with EA-induced intestinal anaphylaxis. Conclusions: Release of ETs in the intestinal mucosa increased in sensitized animals after EA challenge. Ets may play a significant role in the development of intestinal anaphylaxis via an ET(A) receptor.

Original languageEnglish
Pages (from-to)348-356
Number of pages9
JournalGastroenterology
Volume115
Issue number2
DOIs
Publication statusPublished - 1998

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Endothelins
Anaphylaxis
Ovum
Albumins
Histamine Release
Endothelin-3
Endothelin-1
Intestinal Mucosa
Water
Serum
Endothelin A Receptors
Messenger RNA
Food Hypersensitivity
Intraperitoneal Injections
Sprague Dawley Rats
Chickens
Mucous Membrane
Antigens
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Shigematsu, T., Miura, S., Hirokawa, M., Hokari, R., Higuchi, H., Tsuzuki, Y., ... Ishii, H. (1998). Endothelins promote egg albumin-induced intestinal anaphylaxis in rats. Gastroenterology, 115(2), 348-356. https://doi.org/10.1016/S0016-5085(98)70201-X

Endothelins promote egg albumin-induced intestinal anaphylaxis in rats. / Shigematsu, T.; Miura, S.; Hirokawa, M.; Hokari, R.; Higuchi, H.; Tsuzuki, Y.; Kimura, H.; Nakatsumi, R. C.; Serizawa, H.; Saito, Hidetsugu; Ishii, H.

In: Gastroenterology, Vol. 115, No. 2, 1998, p. 348-356.

Research output: Contribution to journalArticle

Shigematsu, T, Miura, S, Hirokawa, M, Hokari, R, Higuchi, H, Tsuzuki, Y, Kimura, H, Nakatsumi, RC, Serizawa, H, Saito, H & Ishii, H 1998, 'Endothelins promote egg albumin-induced intestinal anaphylaxis in rats', Gastroenterology, vol. 115, no. 2, pp. 348-356. https://doi.org/10.1016/S0016-5085(98)70201-X
Shigematsu T, Miura S, Hirokawa M, Hokari R, Higuchi H, Tsuzuki Y et al. Endothelins promote egg albumin-induced intestinal anaphylaxis in rats. Gastroenterology. 1998;115(2):348-356. https://doi.org/10.1016/S0016-5085(98)70201-X
Shigematsu, T. ; Miura, S. ; Hirokawa, M. ; Hokari, R. ; Higuchi, H. ; Tsuzuki, Y. ; Kimura, H. ; Nakatsumi, R. C. ; Serizawa, H. ; Saito, Hidetsugu ; Ishii, H. / Endothelins promote egg albumin-induced intestinal anaphylaxis in rats. In: Gastroenterology. 1998 ; Vol. 115, No. 2. pp. 348-356.
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abstract = "Background and Aims: The basic mechanisms of food allergies are still unknown. The aims of this study were to investigate whether endothelins (ETs) in the intestinal mucosa are involved in the pathogenesis of intestinal anaphylaxis. Methods: Sprague-Dawley rats were sensitized to chicken egg albumin (EA) by intraperitoneal injection. Fourteen days after sensitization, EA was administered in the jejunal segments to induce intestinal anaphylaxis. Net water outflux and histamine release into loops and serum concentrations of rat mast cell protease II (RMCP-II) were determined. ET-1 and ET-3 concentrations in the jejunal mucosa were determined, and expression of the corresponding messenger RNAs was examined by competitive polymerase chain reaction. Results: In sensitized animals, challenge with intraluminal antigen caused a significant increase in net water outflux and histamine release together with an elevation of serum Rmcp-ii concentrations. Mucosal concentrations of ET-1 and ET-3 and expression of their messenger Rnas were significantly increased in sensitized animals after EA challenge. Treatment with an ET(A)-receptor antagonist, but not an ET(B)-receptor antagonist, attenuated the increase in net water outflux, histamine release, and serum Rmcp-ii concentrations in rats with EA-induced intestinal anaphylaxis. Conclusions: Release of ETs in the intestinal mucosa increased in sensitized animals after EA challenge. Ets may play a significant role in the development of intestinal anaphylaxis via an ET(A) receptor.",
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AU - Higuchi, H.

AU - Tsuzuki, Y.

AU - Kimura, H.

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