TY - JOUR
T1 - Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa
AU - Miura, Soichiro
AU - Tsuzuki, Yoshikazu
AU - Kurose, Iwao
AU - Suematsu, Makoto
AU - Shigematsu, Takeharu
AU - Kimura, Hiroyuki
AU - Higuchi, Hajime
AU - Serizawa, Hiroshi
AU - Yagita, Hideo
AU - Okumura, Ko
AU - Granger, D. Neil
AU - Ishii, Hiromasa
PY - 1996/8
Y1 - 1996/8
N2 - Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphold (peyer's patches and nonlymphoid (villus) regions of the small bowel remains poorly understood. We monitored the migration of fluorescence-labeled T lymphocytes into normal and lipopolysaccharide (lps)-inflamed rat intestinal microvessels using intravital microscopy. In Peyer's patches, T lymphocytes selectively adhered to postcapillary venules, although such selectivity was not observed in submucosal venules of villi. T lymphocytes exhibited rolling behavior followed by firm adhesion in microvessels of both the Peyer's patches and the villi, with both types of adhesive interaction being mediated by α4- integrins. The enhanced rolling and adherence of lymphocytes observed in Peyer's patches and submucosal venules of villi of Lps-treated rats were preceded by a reduction in shear rate and were mediaterl largely by α4- integrins and partly by β2-integrins. In capillaries of intestinal mucosa, lymphocyte adherence occurred without rolling and was independent of α4- integrins. Lps also significantly increased adherence of lymphocytes to villus capillaries, which was not mediated by either α4- or β2-integrin. These observations demonstrate significant heterogeneity of lymphocyte- endothelial cell interactions within different regions of the intestinal mucosa.
AB - Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphold (peyer's patches and nonlymphoid (villus) regions of the small bowel remains poorly understood. We monitored the migration of fluorescence-labeled T lymphocytes into normal and lipopolysaccharide (lps)-inflamed rat intestinal microvessels using intravital microscopy. In Peyer's patches, T lymphocytes selectively adhered to postcapillary venules, although such selectivity was not observed in submucosal venules of villi. T lymphocytes exhibited rolling behavior followed by firm adhesion in microvessels of both the Peyer's patches and the villi, with both types of adhesive interaction being mediated by α4- integrins. The enhanced rolling and adherence of lymphocytes observed in Peyer's patches and submucosal venules of villi of Lps-treated rats were preceded by a reduction in shear rate and were mediaterl largely by α4- integrins and partly by β2-integrins. In capillaries of intestinal mucosa, lymphocyte adherence occurred without rolling and was independent of α4- integrins. Lps also significantly increased adherence of lymphocytes to villus capillaries, which was not mediated by either α4- or β2-integrin. These observations demonstrate significant heterogeneity of lymphocyte- endothelial cell interactions within different regions of the intestinal mucosa.
KW - lymphocyte adhesion
KW - lymphocyte rolling
KW - α-integrin
KW - β-integrin
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U2 - 10.1152/ajpgi.1996.271.2.g282
DO - 10.1152/ajpgi.1996.271.2.g282
M3 - Article
C2 - 8770044
AN - SCOPUS:16044368890
VL - 271
SP - G282-G292
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 2 34-2
ER -