Abstract
Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphold (peyer's patches and nonlymphoid (villus) regions of the small bowel remains poorly understood. We monitored the migration of fluorescence-labeled T lymphocytes into normal and lipopolysaccharide (lps)-inflamed rat intestinal microvessels using intravital microscopy. In Peyer's patches, T lymphocytes selectively adhered to postcapillary venules, although such selectivity was not observed in submucosal venules of villi. T lymphocytes exhibited rolling behavior followed by firm adhesion in microvessels of both the Peyer's patches and the villi, with both types of adhesive interaction being mediated by α4- integrins. The enhanced rolling and adherence of lymphocytes observed in Peyer's patches and submucosal venules of villi of Lps-treated rats were preceded by a reduction in shear rate and were mediaterl largely by α4- integrins and partly by β2-integrins. In capillaries of intestinal mucosa, lymphocyte adherence occurred without rolling and was independent of α4- integrins. Lps also significantly increased adherence of lymphocytes to villus capillaries, which was not mediated by either α4- or β2-integrin. These observations demonstrate significant heterogeneity of lymphocyte- endothelial cell interactions within different regions of the intestinal mucosa.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
| Volume | 271 |
| Issue number | 2 34-2 |
| Publication status | Published - 1996 Aug |
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Keywords
- α-integrin
- β-integrin
- lymphocyte adhesion
- lymphocyte rolling
ASJC Scopus subject areas
- Gastroenterology
- Physiology
- Physiology (medical)
Cite this
Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa. / Miura, Soichiro; Tsuzuki, Yoshikazu; Kurose, Iwao; Suematsu, Makoto; Shigematsu, Takeharu; Kimura, Hiroyuki; Higuchi, Hajime; Serizawa, Hiroshi; Yagita, Hideo; Okumura, Ko; Granger, D. Neil; Ishii, Hiromasa.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 271, No. 2 34-2, 08.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa
AU - Miura, Soichiro
AU - Tsuzuki, Yoshikazu
AU - Kurose, Iwao
AU - Suematsu, Makoto
AU - Shigematsu, Takeharu
AU - Kimura, Hiroyuki
AU - Higuchi, Hajime
AU - Serizawa, Hiroshi
AU - Yagita, Hideo
AU - Okumura, Ko
AU - Granger, D. Neil
AU - Ishii, Hiromasa
PY - 1996/8
Y1 - 1996/8
N2 - Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphold (peyer's patches and nonlymphoid (villus) regions of the small bowel remains poorly understood. We monitored the migration of fluorescence-labeled T lymphocytes into normal and lipopolysaccharide (lps)-inflamed rat intestinal microvessels using intravital microscopy. In Peyer's patches, T lymphocytes selectively adhered to postcapillary venules, although such selectivity was not observed in submucosal venules of villi. T lymphocytes exhibited rolling behavior followed by firm adhesion in microvessels of both the Peyer's patches and the villi, with both types of adhesive interaction being mediated by α4- integrins. The enhanced rolling and adherence of lymphocytes observed in Peyer's patches and submucosal venules of villi of Lps-treated rats were preceded by a reduction in shear rate and were mediaterl largely by α4- integrins and partly by β2-integrins. In capillaries of intestinal mucosa, lymphocyte adherence occurred without rolling and was independent of α4- integrins. Lps also significantly increased adherence of lymphocytes to villus capillaries, which was not mediated by either α4- or β2-integrin. These observations demonstrate significant heterogeneity of lymphocyte- endothelial cell interactions within different regions of the intestinal mucosa.
AB - Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphold (peyer's patches and nonlymphoid (villus) regions of the small bowel remains poorly understood. We monitored the migration of fluorescence-labeled T lymphocytes into normal and lipopolysaccharide (lps)-inflamed rat intestinal microvessels using intravital microscopy. In Peyer's patches, T lymphocytes selectively adhered to postcapillary venules, although such selectivity was not observed in submucosal venules of villi. T lymphocytes exhibited rolling behavior followed by firm adhesion in microvessels of both the Peyer's patches and the villi, with both types of adhesive interaction being mediated by α4- integrins. The enhanced rolling and adherence of lymphocytes observed in Peyer's patches and submucosal venules of villi of Lps-treated rats were preceded by a reduction in shear rate and were mediaterl largely by α4- integrins and partly by β2-integrins. In capillaries of intestinal mucosa, lymphocyte adherence occurred without rolling and was independent of α4- integrins. Lps also significantly increased adherence of lymphocytes to villus capillaries, which was not mediated by either α4- or β2-integrin. These observations demonstrate significant heterogeneity of lymphocyte- endothelial cell interactions within different regions of the intestinal mucosa.
KW - α-integrin
KW - β-integrin
KW - lymphocyte adhesion
KW - lymphocyte rolling
UR - http://www.scopus.com/inward/record.url?scp=16044368890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16044368890&partnerID=8YFLogxK
M3 - Article
C2 - 8770044
AN - SCOPUS:16044368890
VL - 271
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 2 34-2
ER -