Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa

Soichiro Miura, Yoshikazu Tsuzuki, Iwao Kurose, Makoto Suematsu, Takeharu Shigematsu, Hiroyuki Kimura, Hajime Higuchi, Hiroshi Serizawa, Hideo Yagita, Ko Okumura, D. Neil Granger, Hiromasa Ishii

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphold (peyer's patches and nonlymphoid (villus) regions of the small bowel remains poorly understood. We monitored the migration of fluorescence-labeled T lymphocytes into normal and lipopolysaccharide (lps)-inflamed rat intestinal microvessels using intravital microscopy. In Peyer's patches, T lymphocytes selectively adhered to postcapillary venules, although such selectivity was not observed in submucosal venules of villi. T lymphocytes exhibited rolling behavior followed by firm adhesion in microvessels of both the Peyer's patches and the villi, with both types of adhesive interaction being mediated by α4- integrins. The enhanced rolling and adherence of lymphocytes observed in Peyer's patches and submucosal venules of villi of Lps-treated rats were preceded by a reduction in shear rate and were mediaterl largely by α4- integrins and partly by β2-integrins. In capillaries of intestinal mucosa, lymphocyte adherence occurred without rolling and was independent of α4- integrins. Lps also significantly increased adherence of lymphocytes to villus capillaries, which was not mediated by either α4- or β2-integrin. These observations demonstrate significant heterogeneity of lymphocyte- endothelial cell interactions within different regions of the intestinal mucosa.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume271
Issue number2 34-2
Publication statusPublished - 1996 Aug

Fingerprint

Peyer's Patches
Endotoxins
Mucous Membrane
Integrins
Lymphocytes
Venules
Microvessels
Intestinal Mucosa
T-Lymphocytes
Cell Communication
Endothelial Cells
Adhesives
Lipopolysaccharides
Fluorescence

Keywords

  • α-integrin
  • β-integrin
  • lymphocyte adhesion
  • lymphocyte rolling

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa. / Miura, Soichiro; Tsuzuki, Yoshikazu; Kurose, Iwao; Suematsu, Makoto; Shigematsu, Takeharu; Kimura, Hiroyuki; Higuchi, Hajime; Serizawa, Hiroshi; Yagita, Hideo; Okumura, Ko; Granger, D. Neil; Ishii, Hiromasa.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 271, No. 2 34-2, 08.1996.

Research output: Contribution to journalArticle

Miura, S, Tsuzuki, Y, Kurose, I, Suematsu, M, Shigematsu, T, Kimura, H, Higuchi, H, Serizawa, H, Yagita, H, Okumura, K, Granger, DN & Ishii, H 1996, 'Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 271, no. 2 34-2.
Miura, Soichiro ; Tsuzuki, Yoshikazu ; Kurose, Iwao ; Suematsu, Makoto ; Shigematsu, Takeharu ; Kimura, Hiroyuki ; Higuchi, Hajime ; Serizawa, Hiroshi ; Yagita, Hideo ; Okumura, Ko ; Granger, D. Neil ; Ishii, Hiromasa. / Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1996 ; Vol. 271, No. 2 34-2.
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