Enhanced angiogenesis by gelatin hydrogels incorporating basic fibroblast growth factor in rabbit model of hind limb ischemia

Kazuhiko Doi, Tadashi Ikeda, Akira Marui, Toshihiro Kushibiki, Yoshio Arai, Keiichi Hirose, Yoshiharu Soga, Atsushi Iwakura, Koji Ueyama, Kenichi Yamahara, Hiroshi Itoh, Kazunobu Nishimura, Yasuhiko Tabata, Masashi Komeda

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Recently we have developed new sustained release system of basic fibroblast growth factor (bFGF) using gelatin hydrogel as a carrier. Using this system, we examined the effect of topical sustained release of bFGF on angiogenesis and tissue blood perfusion in a rabbit model of hind limb ischemia. Thirty-two rabbits underwent excision of right femoral artery under general anesthesia. Two weeks later the rabbits were randomized into four groups (n = 8 each): no treatment, intramuscular injection of gelatin hydrogel alone, and intramuscular injection of gelatin hydrogel incorporating 30 μg and 100 μg of bFGF. Four weeks after each treatment, selective angiography, tissue blood flowmetry using laser Doppler perfusion imaging, and histological examination of thigh muscle were performed. In groups treated with bFGF incorporating gelatin hydrogel, tissue blood flow, number of arterioles, and vascular density were significantly increased in a dose-dependent manner 4 weeks after the treatment. Serum concentrations of bFGF and vascular endothelial growth factor were not elevated 4 weeks after the treatment. In conclusion, sustained release of bFGF using gelatin hydrogel augmented angiogenesis and improved tissue blood flow after excision of the femoral artery.

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalHeart and vessels
Volume22
Issue number2
DOIs
Publication statusPublished - 2007 Mar 1
Externally publishedYes

Keywords

  • Angiogenesis
  • Drug delivery
  • Growth factor
  • Limb ischemia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Enhanced angiogenesis by gelatin hydrogels incorporating basic fibroblast growth factor in rabbit model of hind limb ischemia'. Together they form a unique fingerprint.

  • Cite this

    Doi, K., Ikeda, T., Marui, A., Kushibiki, T., Arai, Y., Hirose, K., Soga, Y., Iwakura, A., Ueyama, K., Yamahara, K., Itoh, H., Nishimura, K., Tabata, Y., & Komeda, M. (2007). Enhanced angiogenesis by gelatin hydrogels incorporating basic fibroblast growth factor in rabbit model of hind limb ischemia. Heart and vessels, 22(2), 104-108. https://doi.org/10.1007/s00380-006-0934-0