Enhanced Angpt1/Tie2 signaling affects the differentiation and long-term repopulation ability of hematopoietic stem cells

Yoshiko Matsumoto Ikushima, Fumio Arai, Yuka Nakamura, Kentaro Hosokawa, Yoshiaki Kubota, Masanori Hirashima, Hirofumi Toyama, Toshio Suda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Angiopoietin-1 (Angpt1) signaling via the Tie2 receptor regulates vascular and hematopoietic systems. To investigate the role of Angpt1-Tie2 signaling in hematopoiesis, we prepared conditionally inducible transgenic (Tg) mice expressing a genetically engineered Angpt1, cartridge oligomeric matrix protein (COMP)-Angpt1. The effects of COMP-Angpt1 overexpression in osteoblasts on hematopoiesis were then investigated by crossing COMP-Angpt1 Tg mice with Col1a1-Cre Tg mice. Interestingly, peripheral blood analyses showed that 4week (wk)-old (but not 8 wk-old) Col1a1-Cre+/COMP-Angpt1+ mice had a lower percentage of circulating B cells and a higher percentage of myeloid cells than Col1a1-Cre-/COMP-Angpt1+ (control) mice. Although there were no significant differences in the immunophenotypic hematopoietic stem and progenitor cell (HSPC) populations between Col1a1-Cre+/COMP-Angpt1+ and control mice, lineage-Sca-1+c-Kit+ (LSK) cells isolated from 8 wk-old Col1a1-Cre+/COMP-Angpt1+ mice showed better long-term bone marrow reconstitution ability. These data indicate that Angpt1-Tie2 signaling affects the differentiation capacity of hematopoietic lineages during development and increases the stem cell activity of HSCs.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume430
Issue number1
DOIs
Publication statusPublished - 2013 Jan 4

Fingerprint

Angiopoietin-1
Hematopoietic Stem Cells
Stem cells
Transgenic Mice
Hematopoiesis
TIE-2 Receptor
Proteins
Hematopoietic System
Cells
Myeloid Cells
Osteoblasts
Blood Vessels
B-Lymphocytes
Stem Cells
Bone Marrow
Bone
Blood
Mouse Angpt1 protein

Keywords

  • Angiopoietin1
  • COMP-Angiopoietin1
  • Hematopoietic stem cells
  • Tie2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Enhanced Angpt1/Tie2 signaling affects the differentiation and long-term repopulation ability of hematopoietic stem cells. / Ikushima, Yoshiko Matsumoto; Arai, Fumio; Nakamura, Yuka; Hosokawa, Kentaro; Kubota, Yoshiaki; Hirashima, Masanori; Toyama, Hirofumi; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 430, No. 1, 04.01.2013, p. 20-25.

Research output: Contribution to journalArticle

Ikushima, Yoshiko Matsumoto ; Arai, Fumio ; Nakamura, Yuka ; Hosokawa, Kentaro ; Kubota, Yoshiaki ; Hirashima, Masanori ; Toyama, Hirofumi ; Suda, Toshio. / Enhanced Angpt1/Tie2 signaling affects the differentiation and long-term repopulation ability of hematopoietic stem cells. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 430, No. 1. pp. 20-25.
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