Abstract
Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. In our previous study, we examined the role of STAP-2 in the c-Fms/M-CSF receptor signaling using a murine macrophage tumor cells line, Raw264.7. Overexpression of STAP-2 in Raw264.7 cells markedly suppressed M-CSF-induced activation of extracellular signal regulated kinase and Akt. In addition, Raw264.7 overexpressing STAP-2 affected cell migration in wound-healing process. These results suggest that STAP-2 deficiency influences endogenous c-Fms/M-CSF receptor signaling. Here we show that loss of STAP-2 expression in knockout mouse macrophages results in marked enhancement of the c-Fms/M-CSF receptor signaling and wound-healing process. We therefore propose that STAP-2 acts as an endogenous regulator in normal macrophages functions.
| Original language | English |
|---|---|
| Pages (from-to) | 1790-1793 |
| Number of pages | 4 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 31 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2008 Sep |
| Externally published | Yes |
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Keywords
- C-Fms
- M-CSF
- Macrophage
- Signal-transducing adaptor protein-2
- Wound-healing
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Enhanced c-Fms/M-CSF receptor signaling and wound-healing process in bone marrow-derived macrophages of signal-transducing adaptor protein-2 (STAP-2) deficient mice. / Ikeda, Osamu; Sekine, Yuichi; Muromoto, Ryuta; Ohbayashi, Norihiko; Yoshimura, Akihiko; Matsuda, Tadashi.
In: Biological and Pharmaceutical Bulletin, Vol. 31, No. 9, 09.2008, p. 1790-1793.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced c-Fms/M-CSF receptor signaling and wound-healing process in bone marrow-derived macrophages of signal-transducing adaptor protein-2 (STAP-2) deficient mice
AU - Ikeda, Osamu
AU - Sekine, Yuichi
AU - Muromoto, Ryuta
AU - Ohbayashi, Norihiko
AU - Yoshimura, Akihiko
AU - Matsuda, Tadashi
PY - 2008/9
Y1 - 2008/9
N2 - Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. In our previous study, we examined the role of STAP-2 in the c-Fms/M-CSF receptor signaling using a murine macrophage tumor cells line, Raw264.7. Overexpression of STAP-2 in Raw264.7 cells markedly suppressed M-CSF-induced activation of extracellular signal regulated kinase and Akt. In addition, Raw264.7 overexpressing STAP-2 affected cell migration in wound-healing process. These results suggest that STAP-2 deficiency influences endogenous c-Fms/M-CSF receptor signaling. Here we show that loss of STAP-2 expression in knockout mouse macrophages results in marked enhancement of the c-Fms/M-CSF receptor signaling and wound-healing process. We therefore propose that STAP-2 acts as an endogenous regulator in normal macrophages functions.
AB - Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. In our previous study, we examined the role of STAP-2 in the c-Fms/M-CSF receptor signaling using a murine macrophage tumor cells line, Raw264.7. Overexpression of STAP-2 in Raw264.7 cells markedly suppressed M-CSF-induced activation of extracellular signal regulated kinase and Akt. In addition, Raw264.7 overexpressing STAP-2 affected cell migration in wound-healing process. These results suggest that STAP-2 deficiency influences endogenous c-Fms/M-CSF receptor signaling. Here we show that loss of STAP-2 expression in knockout mouse macrophages results in marked enhancement of the c-Fms/M-CSF receptor signaling and wound-healing process. We therefore propose that STAP-2 acts as an endogenous regulator in normal macrophages functions.
KW - C-Fms
KW - M-CSF
KW - Macrophage
KW - Signal-transducing adaptor protein-2
KW - Wound-healing
UR - http://www.scopus.com/inward/record.url?scp=51449083103&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51449083103&partnerID=8YFLogxK
U2 - 10.1248/bpb.31.1790
DO - 10.1248/bpb.31.1790
M3 - Article
VL - 31
SP - 1790
EP - 1793
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 9
ER -