Enhanced c-Fms/M-CSF receptor signaling and wound-healing process in bone marrow-derived macrophages of signal-transducing adaptor protein-2 (STAP-2) deficient mice

Osamu Ikeda, Yuichi Sekine, Ryuta Muromoto, Norihiko Ohbayashi, Akihiko Yoshimura, Tadashi Matsuda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. In our previous study, we examined the role of STAP-2 in the c-Fms/M-CSF receptor signaling using a murine macrophage tumor cells line, Raw264.7. Overexpression of STAP-2 in Raw264.7 cells markedly suppressed M-CSF-induced activation of extracellular signal regulated kinase and Akt. In addition, Raw264.7 overexpressing STAP-2 affected cell migration in wound-healing process. These results suggest that STAP-2 deficiency influences endogenous c-Fms/M-CSF receptor signaling. Here we show that loss of STAP-2 expression in knockout mouse macrophages results in marked enhancement of the c-Fms/M-CSF receptor signaling and wound-healing process. We therefore propose that STAP-2 acts as an endogenous regulator in normal macrophages functions.

Original languageEnglish
Pages (from-to)1790-1793
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume31
Issue number9
DOIs
Publication statusPublished - 2008 Sep
Externally publishedYes

Fingerprint

Signal Transducing Adaptor Proteins
Macrophage Colony-Stimulating Factor Receptors
Wound Healing
Macrophages
Receptor-Interacting Protein Serine-Threonine Kinases
Protein Deficiency
Macrophage Colony-Stimulating Factor
Extracellular Signal-Regulated MAP Kinases
Tumor Cell Line
Knockout Mice
Cell Movement

Keywords

  • C-Fms
  • M-CSF
  • Macrophage
  • Signal-transducing adaptor protein-2
  • Wound-healing

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Enhanced c-Fms/M-CSF receptor signaling and wound-healing process in bone marrow-derived macrophages of signal-transducing adaptor protein-2 (STAP-2) deficient mice. / Ikeda, Osamu; Sekine, Yuichi; Muromoto, Ryuta; Ohbayashi, Norihiko; Yoshimura, Akihiko; Matsuda, Tadashi.

In: Biological and Pharmaceutical Bulletin, Vol. 31, No. 9, 09.2008, p. 1790-1793.

Research output: Contribution to journalArticle

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