Enhanced cortical blood flow during selective stimulation of sensory or postganglionic parasympathetic cerebrovascular nerve fibers in rats

Possible role of putative transmitters

N. Suzuki, J. E. Hardebo, C. Owman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to clarify the functional role of the cerebrovascular parasympathetic and sensory nerves on the vessel tone in vivo, and the transmitters responsible for such an effect, brain cortical microvascular blood flow was measured in rats during electrical stimulation of these particular postganglionic parasympathetic and sensory fibers. Their respective central connections were cut prior to stimulation, to avoid cerebral flow changes secondary to activation of central structures. Relative changes in microvascular blood flow was continuously recorded by a laser-Doppler flowmeter system under α-chloralose or ketamine anesthesia. Stimulation of the postganglionic parasympathetic fibers induced a marked increase in the cortical blood flow on the ipsilateral side, whereas no change was observed on the contralateral side. Atropine and scopolamine, applied locally or systemically in effective concentrations, failed to alter the flow increase upon parasympathetic stimulation. The strong vasodilator vasoactive intestinal polypeptide found in these nerves remains as a possible transmitter responsible for the flow increase, although a proposed antagonist tested did not attenuate the response. The modest flow increase seen ipsilaterally upon stimulation of the sensory nerve fibers is likely to be caused by antidromic release of transmitter in pain fibers. Antagonists to substance P did not affect the response. The strong vasodilator in these nerves, calcitonin gene-related peptide, is a possible candidate responsible for the flow increase.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalBiomedical Research
Volume11
Issue number1
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Postganglionic Parasympathetic Fibers
Nerve Fibers
Rats
Transmitters
Blood
Vasodilator Agents
Fibers
Flowmeters
Chloralose
Scopolamine Hydrobromide
Calcitonin Gene-Related Peptide
Vasoactive Intestinal Peptide
Ketamine
Substance P
Atropine
Electric Stimulation
Lasers
Anesthesia
Pain
Brain

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Enhanced cortical blood flow during selective stimulation of sensory or postganglionic parasympathetic cerebrovascular nerve fibers in rats: Possible role of putative transmitters",
abstract = "In order to clarify the functional role of the cerebrovascular parasympathetic and sensory nerves on the vessel tone in vivo, and the transmitters responsible for such an effect, brain cortical microvascular blood flow was measured in rats during electrical stimulation of these particular postganglionic parasympathetic and sensory fibers. Their respective central connections were cut prior to stimulation, to avoid cerebral flow changes secondary to activation of central structures. Relative changes in microvascular blood flow was continuously recorded by a laser-Doppler flowmeter system under α-chloralose or ketamine anesthesia. Stimulation of the postganglionic parasympathetic fibers induced a marked increase in the cortical blood flow on the ipsilateral side, whereas no change was observed on the contralateral side. Atropine and scopolamine, applied locally or systemically in effective concentrations, failed to alter the flow increase upon parasympathetic stimulation. The strong vasodilator vasoactive intestinal polypeptide found in these nerves remains as a possible transmitter responsible for the flow increase, although a proposed antagonist tested did not attenuate the response. The modest flow increase seen ipsilaterally upon stimulation of the sensory nerve fibers is likely to be caused by antidromic release of transmitter in pain fibers. Antagonists to substance P did not affect the response. The strong vasodilator in these nerves, calcitonin gene-related peptide, is a possible candidate responsible for the flow increase.",
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AU - Owman, C.

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AB - In order to clarify the functional role of the cerebrovascular parasympathetic and sensory nerves on the vessel tone in vivo, and the transmitters responsible for such an effect, brain cortical microvascular blood flow was measured in rats during electrical stimulation of these particular postganglionic parasympathetic and sensory fibers. Their respective central connections were cut prior to stimulation, to avoid cerebral flow changes secondary to activation of central structures. Relative changes in microvascular blood flow was continuously recorded by a laser-Doppler flowmeter system under α-chloralose or ketamine anesthesia. Stimulation of the postganglionic parasympathetic fibers induced a marked increase in the cortical blood flow on the ipsilateral side, whereas no change was observed on the contralateral side. Atropine and scopolamine, applied locally or systemically in effective concentrations, failed to alter the flow increase upon parasympathetic stimulation. The strong vasodilator vasoactive intestinal polypeptide found in these nerves remains as a possible transmitter responsible for the flow increase, although a proposed antagonist tested did not attenuate the response. The modest flow increase seen ipsilaterally upon stimulation of the sensory nerve fibers is likely to be caused by antidromic release of transmitter in pain fibers. Antagonists to substance P did not affect the response. The strong vasodilator in these nerves, calcitonin gene-related peptide, is a possible candidate responsible for the flow increase.

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