TY - JOUR
T1 - Enhanced enzyme stability and gas utilization by microbubble aeration applying microporous aerators
AU - Thomas, Benjamin
AU - Ohde, Daniel
AU - Matthes, Simon
AU - Bubenheim, Paul
AU - Terasaka, Koichi
AU - Schlüter, Michael
AU - Liese, Andreas
N1 - Funding Information:
We are grateful to the Deutsche Forschungsgemeinschaft (DFG) for financial support (DFG, LI 899/10-1 and SCHL 617/14-1) and to all our cooperation partners especially c-LEcta for the support with the NOX34. We want to thank the lab apprentices Friederike Dellien, Marie Gruber, Mary Huynh, and Huyen Vu for their excellent help in the laboratory. Further we want to thank Zeynep Percin for carried out the bubble size measurements and software analysis of the bubble measurement data.
Publisher Copyright:
© 2023 The Royal Society of Chemistry.
PY - 2022
Y1 - 2022
N2 - During the optimization of processes in biocatalysis, the limitations of mass transfer and enzyme stability have to be overcome. To achieve these goals, aeration with microbubbles is advantageous by providing favorable properties, which include high gas utility, low-pressure drop, and reduced foam formation. In this study, the influences of microbubble, classic sub-millibubble and macrobubble aeration on enzyme stability and gas utility are compared. Using five enzymes, the half-life was investigated by comparing the microbubble aeration with a 2 μm porous sintered frit to the macrobubble aeration with a 5 mm open pipe, both adjusted to provide a constant kLa of 160 h−1. Furthermore, the half-life of a glucose oxidase was investigated by variation of the kLa in the range of 40 to 350 h−1 and the mode of the aeration system comparing the 2 μm and 10 μm sintered frit as well as the 5 mm open pipe. In both experimental set-ups, it was demonstrated that the aeration with microbubbles using the 2 μm sintered frit was beneficial for reaching high levels of enzyme stability. From this, we discovered that the interfacial area renewal rate plays an important role in the process of enzyme deactivation, which changes depending on the aeration mode.
AB - During the optimization of processes in biocatalysis, the limitations of mass transfer and enzyme stability have to be overcome. To achieve these goals, aeration with microbubbles is advantageous by providing favorable properties, which include high gas utility, low-pressure drop, and reduced foam formation. In this study, the influences of microbubble, classic sub-millibubble and macrobubble aeration on enzyme stability and gas utility are compared. Using five enzymes, the half-life was investigated by comparing the microbubble aeration with a 2 μm porous sintered frit to the macrobubble aeration with a 5 mm open pipe, both adjusted to provide a constant kLa of 160 h−1. Furthermore, the half-life of a glucose oxidase was investigated by variation of the kLa in the range of 40 to 350 h−1 and the mode of the aeration system comparing the 2 μm and 10 μm sintered frit as well as the 5 mm open pipe. In both experimental set-ups, it was demonstrated that the aeration with microbubbles using the 2 μm sintered frit was beneficial for reaching high levels of enzyme stability. From this, we discovered that the interfacial area renewal rate plays an important role in the process of enzyme deactivation, which changes depending on the aeration mode.
UR - http://www.scopus.com/inward/record.url?scp=85146314772&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85146314772&partnerID=8YFLogxK
U2 - 10.1039/d2cy01761j
DO - 10.1039/d2cy01761j
M3 - Article
AN - SCOPUS:85146314772
SN - 2044-4753
JO - Catalysis Science and Technology
JF - Catalysis Science and Technology
ER -