Enhanced epithelial-mesenchymal transition-like phenotype in N-acetylglucosaminyltransferase V transgenic mouse skin promotes wound healing

Mika Terao, Akiko Ishikawa, Susumu Nakahara, Akihiro Kimura, Arisa Kato, Kenta Moriwaki, Yoshihiro Kamada, Hiroyuki Murota, Naoyuki Taniguchi, Ichiro Katayama, Eiji Miyoshi

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48 Citations (Scopus)

Abstract

N-Acetylglucosaminyltransferase V (GnT-V) catalyzes the β1,6 branching of N-acetylglucosamine on N-glycans. GnT-V expression is elevated during malignant transformation in various types of cancer. However, the mechanism by which GnT-V promotes cancer progression is unclear. To characterize the biological significance of GnT-V, we established GnT-V transgenic (Tg) mice, in which GnT-V is regulated by a β-actin promoter. Nospontaneous cancer was detected in any organs of the GnT-V Tg mice. However, GnT-V expression was up-regulated in GnT-V Tg mouse skin, and cultured keratinocytes derived from these mice showed enhanced migration, which was associated with changes in E-cadherin localization and epithelial-mesenchymal transition (EMT). Further, EMT-associated factors snail, twist, and N-cadherin were up-regulated, and cutaneous wound healing was accelerated in vivo. We further investigated the detailed mechanisms of EMT by assessing EGF signaling and found up-regulated EGF receptor signaling in GnT-V Tg mouse keratinocytes. These findings indicate that GnT-V overexpression promotes EMT and keratinocyte migration in part through enhanced EGF receptor signaling.

Original languageEnglish
Pages (from-to)28303-28311
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number32
DOIs
Publication statusPublished - 2011 Aug 12

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Terao, M., Ishikawa, A., Nakahara, S., Kimura, A., Kato, A., Moriwaki, K., Kamada, Y., Murota, H., Taniguchi, N., Katayama, I., & Miyoshi, E. (2011). Enhanced epithelial-mesenchymal transition-like phenotype in N-acetylglucosaminyltransferase V transgenic mouse skin promotes wound healing. Journal of Biological Chemistry, 286(32), 28303-28311. https://doi.org/10.1074/jbc.M111.220376