Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen

Kazuki Santa, Kenji Watanabe, Tsutomu Nakada, Hiroyuki Kato, Sonoko Habu, Shunichiro Kubota

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19+ B cell numbers overturned to CD4+ helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19+ B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.

Original languageEnglish
Pages (from-to)5-12
Number of pages8
JournalImmunology Letters
Volume85
Issue number1
DOIs
Publication statusPublished - 2003 Jan 2

Keywords

  • Atopic dermatitis (AD)
  • B7.1 (CD80)
  • B7.2(CD86)
  • House dust mite antigen
  • IFN-γ
  • IL-4
  • IgE
  • Th1/Th2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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