Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen

Kazuki Santa, Kenji Watanabe, Tsutomu Nakada, Hiroyuki Kato, Sonoko Habu, Shunichiro Kubota

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19+ B cell numbers overturned to CD4+ helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19+ B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.

Original languageEnglish
Pages (from-to)5-12
Number of pages8
JournalImmunology Letters
Volume85
Issue number1
DOIs
Publication statusPublished - 2003 Jan 2

Fingerprint

Dermatophagoides Antigens
Atopic Dermatitis
B-Lymphocytes
Th1-Th2 Balance
Helper-Inducer T-Lymphocytes
Skin Diseases
Granulocytes
Interleukin-4
Allergens
Immunoglobulin E
Monocytes
Cell Count
Lymph Nodes
Antigens
Messenger RNA
Skin
Serum

Keywords

  • Atopic dermatitis (AD)
  • B7.1 (CD80)
  • B7.2(CD86)
  • House dust mite antigen
  • IFN-γ
  • IgE
  • IL-4
  • Th1/Th2

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen. / Santa, Kazuki; Watanabe, Kenji; Nakada, Tsutomu; Kato, Hiroyuki; Habu, Sonoko; Kubota, Shunichiro.

In: Immunology Letters, Vol. 85, No. 1, 02.01.2003, p. 5-12.

Research output: Contribution to journalArticle

Santa, Kazuki ; Watanabe, Kenji ; Nakada, Tsutomu ; Kato, Hiroyuki ; Habu, Sonoko ; Kubota, Shunichiro. / Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen. In: Immunology Letters. 2003 ; Vol. 85, No. 1. pp. 5-12.
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