Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen

Kazuki Santa; Kenji Watanabe; Tsutomu Nakada; Hiroyuki Kato; Sonoko Habu; Shunichiro Kubota

doi:10.1016/S0165-2478(02)00196-7

Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen

Kazuki Santa, Kenji Watanabe, Tsutomu Nakada, Hiroyuki Kato, Sonoko Habu, Shunichiro Kubota

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19⁺ B cell numbers overturned to CD4⁺ helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19⁺ B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.

Original language	English
Pages (from-to)	5-12
Number of pages	8
Journal	Immunology Letters
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/S0165-2478(02)00196-7
Publication status	Published - 2003 Jan 2
Externally published	Yes

Keywords

Atopic dermatitis (AD)
B7.1 (CD80)
B7.2(CD86)
House dust mite antigen
IFN-γ
IL-4
IgE
Th1/Th2

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0165-2478(02)00196-7

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title = "Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen",

abstract = "House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19+ B cell numbers overturned to CD4+ helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19+ B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.",

keywords = "Atopic dermatitis (AD), B7.1 (CD80), B7.2(CD86), House dust mite antigen, IFN-γ, IL-4, IgE, Th1/Th2",

author = "Kazuki Santa and Kenji Watanabe and Tsutomu Nakada and Hiroyuki Kato and Sonoko Habu and Shunichiro Kubota",

note = "Funding Information: This work was supported by grants from the Ministry of Education, Science, Sports and Culture, Japan. (Project no. 11770257) 1999–2000. The authors would like to thanks for Dr Masamichi Aikawa and Dr Yamaji Nakano.",

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T1 - Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen

AU - Santa, Kazuki

AU - Watanabe, Kenji

AU - Nakada, Tsutomu

AU - Kato, Hiroyuki

AU - Habu, Sonoko

AU - Kubota, Shunichiro

N1 - Funding Information: This work was supported by grants from the Ministry of Education, Science, Sports and Culture, Japan. (Project no. 11770257) 1999–2000. The authors would like to thanks for Dr Masamichi Aikawa and Dr Yamaji Nakano.

PY - 2003/1/2

Y1 - 2003/1/2

N2 - House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19+ B cell numbers overturned to CD4+ helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19+ B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.

AB - House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19+ B cell numbers overturned to CD4+ helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19+ B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.

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KW - B7.2(CD86)

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KW - IL-4

KW - IgE

KW - Th1/Th2

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Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen

Abstract

Keywords

ASJC Scopus subject areas

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