Enhanced expression of telomerase activity in thymoma and thymic carcinoma tissues: A clinicopathologic study

Masazumi Watanabe, Shan Kang Yu, Makoto Sawafuji, Masafumi Kawamura, Hirohisa Horinouchi, Makio Mukai, Koichi Kobayashi

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Abstract

BACKGROUND. Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. Because most malignant cells and reproductive cells have telomerase activity, which elongates telomeric DNA, telomerase may play important roles in unlimited cell division acquisition of the malignant phenotype. The current study examined the relation of telomerase activity in thymoma and thymic carcinoma with the clinicopathologic features of these lesions. METHODS. Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol assay. Paraffin sections of tumor were immunostained by MIC2 antibody, a marker of immature T cells. RESULTS. Telomerase activity was detected in all thymic epithelial tumors. The activity (mean ± SD; unit per μg protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) (431.8 ± 400.1 vs. 68.8 ± 39.8; P < 0.01). Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 ± 39.2, n = 47), studied as a control (P < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (P = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (P = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (P = 0.045). MIC2-positive lymphocytes were identified in all cases of thymoma (n = 12). In contrast, lymphocytes infiltrating thymic carcinoma did not react with MIC2. CONCLUSIONS. In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.

Original languageEnglish
Pages (from-to)240-244
Number of pages5
JournalCancer
Volume94
Issue number1
DOIs
Publication statusPublished - 2002 Jan 1

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Thymoma
Telomerase
Neoplasms
Lymphocytes
T-Lymphocytes
Phenotype
Nucleoproteins
Cell Division
Paraffin

Keywords

  • Mediastinal tumor
  • MIC2
  • Telomerase
  • Thymic carcinoma
  • Thymoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Watanabe, M., Yu, S. K., Sawafuji, M., Kawamura, M., Horinouchi, H., Mukai, M., & Kobayashi, K. (2002). Enhanced expression of telomerase activity in thymoma and thymic carcinoma tissues: A clinicopathologic study. Cancer, 94(1), 240-244. https://doi.org/10.1002/cncr.10194

Enhanced expression of telomerase activity in thymoma and thymic carcinoma tissues : A clinicopathologic study. / Watanabe, Masazumi; Yu, Shan Kang; Sawafuji, Makoto; Kawamura, Masafumi; Horinouchi, Hirohisa; Mukai, Makio; Kobayashi, Koichi.

In: Cancer, Vol. 94, No. 1, 01.01.2002, p. 240-244.

Research output: Contribution to journalArticle

Watanabe, M, Yu, SK, Sawafuji, M, Kawamura, M, Horinouchi, H, Mukai, M & Kobayashi, K 2002, 'Enhanced expression of telomerase activity in thymoma and thymic carcinoma tissues: A clinicopathologic study', Cancer, vol. 94, no. 1, pp. 240-244. https://doi.org/10.1002/cncr.10194
Watanabe M, Yu SK, Sawafuji M, Kawamura M, Horinouchi H, Mukai M et al. Enhanced expression of telomerase activity in thymoma and thymic carcinoma tissues: A clinicopathologic study. Cancer. 2002 Jan 1;94(1):240-244. https://doi.org/10.1002/cncr.10194
Watanabe, Masazumi ; Yu, Shan Kang ; Sawafuji, Makoto ; Kawamura, Masafumi ; Horinouchi, Hirohisa ; Mukai, Makio ; Kobayashi, Koichi. / Enhanced expression of telomerase activity in thymoma and thymic carcinoma tissues : A clinicopathologic study. In: Cancer. 2002 ; Vol. 94, No. 1. pp. 240-244.
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abstract = "BACKGROUND. Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. Because most malignant cells and reproductive cells have telomerase activity, which elongates telomeric DNA, telomerase may play important roles in unlimited cell division acquisition of the malignant phenotype. The current study examined the relation of telomerase activity in thymoma and thymic carcinoma with the clinicopathologic features of these lesions. METHODS. Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol assay. Paraffin sections of tumor were immunostained by MIC2 antibody, a marker of immature T cells. RESULTS. Telomerase activity was detected in all thymic epithelial tumors. The activity (mean ± SD; unit per μg protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) (431.8 ± 400.1 vs. 68.8 ± 39.8; P < 0.01). Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 ± 39.2, n = 47), studied as a control (P < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (P = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (P = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (P = 0.045). MIC2-positive lymphocytes were identified in all cases of thymoma (n = 12). In contrast, lymphocytes infiltrating thymic carcinoma did not react with MIC2. CONCLUSIONS. In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.",
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AU - Horinouchi, Hirohisa

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N2 - BACKGROUND. Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. Because most malignant cells and reproductive cells have telomerase activity, which elongates telomeric DNA, telomerase may play important roles in unlimited cell division acquisition of the malignant phenotype. The current study examined the relation of telomerase activity in thymoma and thymic carcinoma with the clinicopathologic features of these lesions. METHODS. Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol assay. Paraffin sections of tumor were immunostained by MIC2 antibody, a marker of immature T cells. RESULTS. Telomerase activity was detected in all thymic epithelial tumors. The activity (mean ± SD; unit per μg protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) (431.8 ± 400.1 vs. 68.8 ± 39.8; P < 0.01). Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 ± 39.2, n = 47), studied as a control (P < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (P = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (P = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (P = 0.045). MIC2-positive lymphocytes were identified in all cases of thymoma (n = 12). In contrast, lymphocytes infiltrating thymic carcinoma did not react with MIC2. CONCLUSIONS. In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.

AB - BACKGROUND. Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. Because most malignant cells and reproductive cells have telomerase activity, which elongates telomeric DNA, telomerase may play important roles in unlimited cell division acquisition of the malignant phenotype. The current study examined the relation of telomerase activity in thymoma and thymic carcinoma with the clinicopathologic features of these lesions. METHODS. Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol assay. Paraffin sections of tumor were immunostained by MIC2 antibody, a marker of immature T cells. RESULTS. Telomerase activity was detected in all thymic epithelial tumors. The activity (mean ± SD; unit per μg protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) (431.8 ± 400.1 vs. 68.8 ± 39.8; P < 0.01). Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 ± 39.2, n = 47), studied as a control (P < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (P = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (P = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (P = 0.045). MIC2-positive lymphocytes were identified in all cases of thymoma (n = 12). In contrast, lymphocytes infiltrating thymic carcinoma did not react with MIC2. CONCLUSIONS. In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.

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KW - Thymic carcinoma

KW - Thymoma

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