Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

Kyoko Tanaka, Masaki Miyazawa, Mikio Mikami, Daisuke Aoki, Kazushige Kiguchi, Masao Iwamori

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Monoclonal antibody YHD-06 generated by immunization with GM2 reacted with gangliosides with GM2-determinant, i.e., GM2, GalNAc-GM1b and GalNAc-GD1a, among which GalNAc-GD1a was characterized as an antigen of autoimmune peripheral neuropathies including Guillain-Barré syndrome. When glycolipids were examined by TLC-immunostaining with YHD-06 in seven human cervical carcinoma-derived cell lines, GM2 was found in all cell lines, amounting to 15.5 % to 57.5 % of total gangliosides. Whereas GalNAc-GD1a was present in three cell lines, amounting to 5.4–17.5 % of total gangliosides, and GalNAc-GM1b in four cell lines in amounts of less than 2 %. The elevated amounts of gangliosides with GM2 determinant were closely correlated with the relative intensities of gene expression of GalNAc transferase, this being characteristic of cervical carcinoma-derived cells. However, in tissues from patients with several histological types of cervical carcinomas, GM3 was ubiquitously expressed in amounts of more than 66 % of total gangliosides, GM2 was expressed in only five of 15 tissues, and both GalNAc-GM1b and GalNAc-GD1a were not even detected in trace amounts. Since GM1 was detected in all tissues in amounts of less than 0.06 μg/mg dried tissue, all cervical carcinoma tissues were revealed to exhibit GM2 synthesis, indicating that enhanced synthesis of gangliosides with GM2 determinant is a characteristic of cultivated cells in vitro. Similarly, although I3SO3-GalCer was not present in the squamous cell carcinoma (SCC) tissues, SCC-derived cells selectively expressed II3SO3-LacCer. Since enhanced synthesis of GM2 has been reported in SV-40 virus-transfected fibroblasts, papilloma virus might be involved in the expression of GM2 in cervical carcinoma-derived cells.

Original languageEnglish
Pages (from-to)745-754
Number of pages10
JournalGlycoconjugate Journal
Volume33
Issue number5
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

G(M2) Ganglioside
Gangliosides
Cells
Tissue
Carcinoma
Cell Line
Viruses
Squamous Cell Carcinoma
Immunization
Simian virus 40
Glycolipids
Papilloma
Peripheral Nervous System Diseases
Fibroblasts
Gene expression
Monoclonal Antibodies
Gene Expression
Antigens
IV(4)-galactosyl-N-acetylganglioside GD1a

Keywords

  • a-series ganglioside
  • Glycolipid
  • Glycosyltransferase
  • Gynecological cancer
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines. / Tanaka, Kyoko; Miyazawa, Masaki; Mikami, Mikio; Aoki, Daisuke; Kiguchi, Kazushige; Iwamori, Masao.

In: Glycoconjugate Journal, Vol. 33, No. 5, 01.10.2016, p. 745-754.

Research output: Contribution to journalArticle

Tanaka, Kyoko ; Miyazawa, Masaki ; Mikami, Mikio ; Aoki, Daisuke ; Kiguchi, Kazushige ; Iwamori, Masao. / Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines. In: Glycoconjugate Journal. 2016 ; Vol. 33, No. 5. pp. 745-754.
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abstract = "Monoclonal antibody YHD-06 generated by immunization with GM2 reacted with gangliosides with GM2-determinant, i.e., GM2, GalNAc-GM1b and GalNAc-GD1a, among which GalNAc-GD1a was characterized as an antigen of autoimmune peripheral neuropathies including Guillain-Barr{\'e} syndrome. When glycolipids were examined by TLC-immunostaining with YHD-06 in seven human cervical carcinoma-derived cell lines, GM2 was found in all cell lines, amounting to 15.5 {\%} to 57.5 {\%} of total gangliosides. Whereas GalNAc-GD1a was present in three cell lines, amounting to 5.4–17.5 {\%} of total gangliosides, and GalNAc-GM1b in four cell lines in amounts of less than 2 {\%}. The elevated amounts of gangliosides with GM2 determinant were closely correlated with the relative intensities of gene expression of GalNAc transferase, this being characteristic of cervical carcinoma-derived cells. However, in tissues from patients with several histological types of cervical carcinomas, GM3 was ubiquitously expressed in amounts of more than 66 {\%} of total gangliosides, GM2 was expressed in only five of 15 tissues, and both GalNAc-GM1b and GalNAc-GD1a were not even detected in trace amounts. Since GM1 was detected in all tissues in amounts of less than 0.06 μg/mg dried tissue, all cervical carcinoma tissues were revealed to exhibit GM2 synthesis, indicating that enhanced synthesis of gangliosides with GM2 determinant is a characteristic of cultivated cells in vitro. Similarly, although I3SO3-GalCer was not present in the squamous cell carcinoma (SCC) tissues, SCC-derived cells selectively expressed II3SO3-LacCer. Since enhanced synthesis of GM2 has been reported in SV-40 virus-transfected fibroblasts, papilloma virus might be involved in the expression of GM2 in cervical carcinoma-derived cells.",
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