Enhanced Fluorescence Using an Optical Interference Mirror Overlaid with Silver Island Film

Takafumi Harashima, Mitsuru Yasuda, Takuo Akimoto

Research output: Contribution to journalArticle

Abstract

Fluorophores overlaid on an optical interference mirror composed of a metal and thin dielectric layer demonstrate enhanced fluorescence. Fluorescence is also enhanced by silver nanostructures such as silver island films, which excite localized surface plasmon resonance. An optical interference mirror surface was overlaid with a silver island film to amplify the fluorescence enhancement. The optimal thickness of the silver island film (100 nm) was evaluated from transmittance and surface roughness measurements. At this thickness, the fluorescence was amplified sixteen-fold. The thickness of the interference layer was optimized at 40 nm providing a one hundred-sixty fold fluorescence enhancement of rhodamine B. However, only a four-fold improvement in sensitivity was achieved for the determination of a labeled streptavidin using biotin immobilized on the silver island film interference mirror.

Original languageEnglish
Pages (from-to)2731-2739
Number of pages9
JournalAnalytical Letters
Volume47
Issue number16
DOIs
Publication statusPublished - 2014 Nov 1
Externally publishedYes

Keywords

  • Biochip
  • Fluorescence enhancement
  • Localized surface plasmon

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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