Enhanced fluorescent DNA microarray using optical interference mirror slide

Mitsuru Yasuda, Takuo Akimoto

Research output: Contribution to journalArticle

Abstract

Enhanced fluorescence using an optical interference mirror (OIM) slide consisting of a metal film and a transparent dielectric thin film is used to improve the sensitivity of a DNA microarray. Twenty-one bases of a probe DNA are immobilized on the OIM slide fabricated with Ag and Al2O3, and 21 bases of target DNA, whose sequence is complementary to that of the probe DNA, are detected. After evaluating the hybridization specificity between the probe DNA and the target DNA, the sensitivity improvement is estimated. As a result, a 50-fold improvement can be achieved compared with a bare glass slide.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalSensors and Materials
Volume30
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes

Keywords

  • DNA microarray
  • Fluorescence enhancement
  • Optical interference

ASJC Scopus subject areas

  • Instrumentation
  • Materials Science(all)

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