Enhanced levels of chemiluminescence and platelet activating factor in urease-positive gastric ulcers

Hidekazu Suzuki, Soichiro Miura, Hiroyuki Imaeda, Masayuki Suzuki, Jing Yan Han, Mikiji Mori, Dai Fukumura, Masaharu Tsuchiya, Hiromasa Ishii

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Abstract

Helicobacter pylori are believed to play an important role in the formation of gastric ulcer in a syndrome characterized by a high urease activity. On the other hand, the production of oxygen radicals and platelet activating factor (paf) is enhanced in gastric ulcers. The present study is designed to investigate the relationship between the different aspects of gastric mucosal injury, urease activity, oxygen radical production, and Paf content in gastric specimens. Biopsy specimens taken from 35 gastric ulcer patients were studied. Urease activity was detected by a rapid urease test (clo). Oxygen radical production was measured as a value of luminol-dependent chemiluminescence (chl) and Paf content was determined by radioimmunoassay in the biopsy samples. The Clo-positive rate was significantly higher in the gastric ulcer group in comparison with that in controls. Chl values and Paf content were significantly increased in gastric ulcers, especially in Clo- positive specimens. The Clo-positive rate, Chl values, and Paf content were also found to be increased at a distant site beyond the ulcer lesions. During the course of macroscopic ulcer healing of Clo-positive cases, the Clo positive level and the Chl values were not significantly decreased, although Paf content was significantly lower. Enhanced oxygen radical and Paf production were observed not only in the ulcer region but also at a distant site from the ulcer in the urease-positive gastric mucosa. The persistent enhancement of Chl values during the healing stage of urease-positive gastric ulcers suggests its involvement in the recurrence of gastric ulcers.

Original languageEnglish
Pages (from-to)449-454
Number of pages6
JournalFree Radical Biology and Medicine
Volume20
Issue number3
DOIs
Publication statusPublished - 1996 Jan 1

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Keywords

  • Free radicals
  • Helicobacter pylori
  • Luminol-dependent chemiluminescence
  • Microcirculation
  • Neutrophil
  • Platelet activating factor
  • Urease

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Suzuki, H., Miura, S., Imaeda, H., Suzuki, M., Han, J. Y., Mori, M., Fukumura, D., Tsuchiya, M., & Ishii, H. (1996). Enhanced levels of chemiluminescence and platelet activating factor in urease-positive gastric ulcers. Free Radical Biology and Medicine, 20(3), 449-454. https://doi.org/10.1016/0891-5849(96)02048-5