Enhanced plasma ghrelin levels in Helicobacter pylori-colonized, interleukin-1-receptor type 1-homozygous knockout (IL-1R1-1-) mice

Yuka Abiko, Hidekazu Suzuki, Tatsuhiro Masaoka, Sachiko Nomura, Kumiko Kurabayashi, Hiroshi Hosoda, Kenji Kangawa, Toshifumi Hibi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aim: Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor, and it plays a role in stimulating the growth hormone secretion, food intake, body weight gain and gastric motility. Eradication of Helicobacter pylori (H pylori) was shown to be associated with increase of the body weight. On the other hand, H pylori infection evokes the release of gastric IL-1β. The present study was designed to investigate the involvement of the gastric IL-1 signal in the ghrelin dynamics in H pylori-colonized mice. Methods: Twelve-week-old female IL-1-receptor type 1-homozygous-knockout mice (IL-1R1-/-) and their wild-type littermates (WT) were orally inoculated with H pylori (Hp group), while other cohorts received oral inoculation of culture medium (Cont group). Thirteen weeks after the inoculation, the mice were examined. The plasma and stomach ghrelin levels and the gastric preproghrelin mRNA were measured. Results: Although the WT mice with H pylori infection showed a significantly decreased body weight as compared with that of the animals without H pylori infection, H pylori infection did not influence the body weight of the IL-1R1-knockout (IL-1R1-/-) mice. In the H pylori-infected IL-1R1-/- mice, the total and active ghrelin levels in the plasma were significantly increased, and the gastric ghrelin level was decreased. No significant differences were noted in the gastric preproghrelin mRNA expression. Conclusion: Ghrelin secretion triggered by H pylori infection might be suppressed by IL-1β, the release of which is also induced by the infection, resulting in the body weight loss of mice with H pylori infection.

Original languageEnglish
Pages (from-to)4148-4153
Number of pages6
JournalWorld Journal of Gastroenterology
Volume11
Issue number27
Publication statusPublished - 2005 Jul 21

Fingerprint

Interleukin-1 Type I Receptors
Ghrelin
Interleukin-1
Helicobacter pylori
Helicobacter Infections
Stomach
Body Weight
Knockout Mice
Interleukin-1 Receptors
Ghrelin Receptor
Messenger RNA
Growth Hormone
Weight Gain
Culture Media
Weight Loss

Keywords

  • Body weight
  • Ghrelin
  • H pylori
  • IL-1
  • Myeloperoxidase

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Enhanced plasma ghrelin levels in Helicobacter pylori-colonized, interleukin-1-receptor type 1-homozygous knockout (IL-1R1-1-) mice. / Abiko, Yuka; Suzuki, Hidekazu; Masaoka, Tatsuhiro; Nomura, Sachiko; Kurabayashi, Kumiko; Hosoda, Hiroshi; Kangawa, Kenji; Hibi, Toshifumi.

In: World Journal of Gastroenterology, Vol. 11, No. 27, 21.07.2005, p. 4148-4153.

Research output: Contribution to journalArticle

Abiko, Y, Suzuki, H, Masaoka, T, Nomura, S, Kurabayashi, K, Hosoda, H, Kangawa, K & Hibi, T 2005, 'Enhanced plasma ghrelin levels in Helicobacter pylori-colonized, interleukin-1-receptor type 1-homozygous knockout (IL-1R1-1-) mice', World Journal of Gastroenterology, vol. 11, no. 27, pp. 4148-4153.
Abiko, Yuka ; Suzuki, Hidekazu ; Masaoka, Tatsuhiro ; Nomura, Sachiko ; Kurabayashi, Kumiko ; Hosoda, Hiroshi ; Kangawa, Kenji ; Hibi, Toshifumi. / Enhanced plasma ghrelin levels in Helicobacter pylori-colonized, interleukin-1-receptor type 1-homozygous knockout (IL-1R1-1-) mice. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 27. pp. 4148-4153.
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abstract = "Aim: Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor, and it plays a role in stimulating the growth hormone secretion, food intake, body weight gain and gastric motility. Eradication of Helicobacter pylori (H pylori) was shown to be associated with increase of the body weight. On the other hand, H pylori infection evokes the release of gastric IL-1β. The present study was designed to investigate the involvement of the gastric IL-1 signal in the ghrelin dynamics in H pylori-colonized mice. Methods: Twelve-week-old female IL-1-receptor type 1-homozygous-knockout mice (IL-1R1-/-) and their wild-type littermates (WT) were orally inoculated with H pylori (Hp group), while other cohorts received oral inoculation of culture medium (Cont group). Thirteen weeks after the inoculation, the mice were examined. The plasma and stomach ghrelin levels and the gastric preproghrelin mRNA were measured. Results: Although the WT mice with H pylori infection showed a significantly decreased body weight as compared with that of the animals without H pylori infection, H pylori infection did not influence the body weight of the IL-1R1-knockout (IL-1R1-/-) mice. In the H pylori-infected IL-1R1-/- mice, the total and active ghrelin levels in the plasma were significantly increased, and the gastric ghrelin level was decreased. No significant differences were noted in the gastric preproghrelin mRNA expression. Conclusion: Ghrelin secretion triggered by H pylori infection might be suppressed by IL-1β, the release of which is also induced by the infection, resulting in the body weight loss of mice with H pylori infection.",
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T1 - Enhanced plasma ghrelin levels in Helicobacter pylori-colonized, interleukin-1-receptor type 1-homozygous knockout (IL-1R1-1-) mice

AU - Abiko, Yuka

AU - Suzuki, Hidekazu

AU - Masaoka, Tatsuhiro

AU - Nomura, Sachiko

AU - Kurabayashi, Kumiko

AU - Hosoda, Hiroshi

AU - Kangawa, Kenji

AU - Hibi, Toshifumi

PY - 2005/7/21

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N2 - Aim: Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor, and it plays a role in stimulating the growth hormone secretion, food intake, body weight gain and gastric motility. Eradication of Helicobacter pylori (H pylori) was shown to be associated with increase of the body weight. On the other hand, H pylori infection evokes the release of gastric IL-1β. The present study was designed to investigate the involvement of the gastric IL-1 signal in the ghrelin dynamics in H pylori-colonized mice. Methods: Twelve-week-old female IL-1-receptor type 1-homozygous-knockout mice (IL-1R1-/-) and their wild-type littermates (WT) were orally inoculated with H pylori (Hp group), while other cohorts received oral inoculation of culture medium (Cont group). Thirteen weeks after the inoculation, the mice were examined. The plasma and stomach ghrelin levels and the gastric preproghrelin mRNA were measured. Results: Although the WT mice with H pylori infection showed a significantly decreased body weight as compared with that of the animals without H pylori infection, H pylori infection did not influence the body weight of the IL-1R1-knockout (IL-1R1-/-) mice. In the H pylori-infected IL-1R1-/- mice, the total and active ghrelin levels in the plasma were significantly increased, and the gastric ghrelin level was decreased. No significant differences were noted in the gastric preproghrelin mRNA expression. Conclusion: Ghrelin secretion triggered by H pylori infection might be suppressed by IL-1β, the release of which is also induced by the infection, resulting in the body weight loss of mice with H pylori infection.

AB - Aim: Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor, and it plays a role in stimulating the growth hormone secretion, food intake, body weight gain and gastric motility. Eradication of Helicobacter pylori (H pylori) was shown to be associated with increase of the body weight. On the other hand, H pylori infection evokes the release of gastric IL-1β. The present study was designed to investigate the involvement of the gastric IL-1 signal in the ghrelin dynamics in H pylori-colonized mice. Methods: Twelve-week-old female IL-1-receptor type 1-homozygous-knockout mice (IL-1R1-/-) and their wild-type littermates (WT) were orally inoculated with H pylori (Hp group), while other cohorts received oral inoculation of culture medium (Cont group). Thirteen weeks after the inoculation, the mice were examined. The plasma and stomach ghrelin levels and the gastric preproghrelin mRNA were measured. Results: Although the WT mice with H pylori infection showed a significantly decreased body weight as compared with that of the animals without H pylori infection, H pylori infection did not influence the body weight of the IL-1R1-knockout (IL-1R1-/-) mice. In the H pylori-infected IL-1R1-/- mice, the total and active ghrelin levels in the plasma were significantly increased, and the gastric ghrelin level was decreased. No significant differences were noted in the gastric preproghrelin mRNA expression. Conclusion: Ghrelin secretion triggered by H pylori infection might be suppressed by IL-1β, the release of which is also induced by the infection, resulting in the body weight loss of mice with H pylori infection.

KW - Body weight

KW - Ghrelin

KW - H pylori

KW - IL-1

KW - Myeloperoxidase

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