Enhanced proliferation of progenitor cells in the subventricular zone and limited neuronal production in the striatum and neocortex of adult macaque monkeys after global cerebral ischemia

Anton B. Tonchev, Tetsumori Yamashima, Kazunobu Sawamoto, Hideyuki Okano

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Cerebral ischemia in adult rodent models increases the proliferation of endogenous neural progenitor cells residing in the subventricular zone along the anterior horn of the lateral ventricle (SVZa) and induces neurogenesis in the postischemic striatum and cortex. Whether the adult primate brain preserves a similar ability in response to an ischemic insult is uncertain. We used the DNA synthesis indicator bromodeoxyuridine (BrdU) to label newly generated cells in adult macaque monkeys and show here that the proliferation of cells with a progenitor phenotype (double positive for BrdU and the markers Musashi1, Nestin, and βIII-tubulin) in SVZa increased during the second week after a 20-min transient global brain ischemia. Subsequent progenitor migration seemed restricted to the rostral migratory stream toward the olfactory bulb and ischemia increased the proportion of adult-generated cells retaining their location in SVZa with a progenitor phenotype. Despite the lack of evidence for progenitor cell migration toward the postischemic striatum or prefrontal neocortex, a small but sustained proportion of BrdU-labeled cells expressed features of postmitotic neurons (positive for the protein NeuN and the transcription factors Tbr1 and Islet1) in these two regions for at least 79 days after ischemia. Taken together, our data suggest an enhanced neurogenic response in the adult primate telencephalon after a cerebral ischemic insult.

Original languageEnglish
Pages (from-to)776-788
Number of pages13
JournalJournal of Neuroscience Research
Volume81
Issue number6
DOIs
Publication statusPublished - 2005 Sep 15

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Lateral Ventricles
Neocortex
Macaca
Bromodeoxyuridine
Brain Ischemia
Haplorhini
Stem Cells
Primates
Ischemia
Phenotype
Nestin
Telencephalon
Olfactory Bulb
Neurogenesis
Tubulin
Horns
Cell Movement
Rodentia
Transcription Factors
Cell Proliferation

Keywords

  • Cerebral ischemia
  • Gliogenesis
  • Monkey
  • Neurogenesis
  • Progenitor cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Enhanced proliferation of progenitor cells in the subventricular zone and limited neuronal production in the striatum and neocortex of adult macaque monkeys after global cerebral ischemia. / Tonchev, Anton B.; Yamashima, Tetsumori; Sawamoto, Kazunobu; Okano, Hideyuki.

In: Journal of Neuroscience Research, Vol. 81, No. 6, 15.09.2005, p. 776-788.

Research output: Contribution to journalArticle

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