Enhanced susceptibility to lipopolysaccharide-induced arthritis and endotoxin shock in interleukin-32 alpha transgenic mice through induction of tumor necrosis factor alpha

Masanori Nakayama, Yasuo Niki, Toshiki Kawasaki, Yuki Takeda, Keisuke Horiuchi, Aya Sasaki, Yasunori Okada, Kazuo Umezawa, Hiroyasu Ikegami, Yoshiaki Toyama, Takeshi Miyamoto

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Abstract

Introduction: The present study assessed the potential functions of interleukin (IL)-32α on inflammatory arthritis and endotoxin shock models using IL-32α transgenic (Tg) mice. The potential signaling pathway for the IL-32-tumor necrosis factor (TNF)α axis was analyzed in vitro.Methods: IL-32α Tg mice were generated under control of a ubiquitous promoter. Two disease models were used to examine in vivo effects of overexpressed IL-32α: Toll-like receptor (TLR) ligand-induced arthritis developed using a single injection of lipopolysaccharide (LPS) or zymosan into the knee joints; and endotoxin shock induced with intraperitoneal injection of LPS and D-galactosamine. TNFα antagonist etanercept was administered simultaneously with LPS in some mice. Using RAW264.7 cells, in vitro effects of exogenous IL-32α on TNFα, IL-6 or macrophage inflammatory protein 2 (MIP-2) production were assessed with or without inhibitors for nuclear factor kappa B (NFκB) or mitogen-activated protein kinase (MAPK).Results: Single injection of LPS, but not zymosan, resulted in development of severe synovitis with substantial articular cartilage degradation in knees of the Tg mice. The expression of TNFα mRNA in inflamed synovia was highly upregulated in the LPS-injected Tg mice. Moreover, the Tg mice were more susceptive to endotoxin-induced lethality than the wild-type control mice 48 hours after LPS challenge; but blockade of TNFα by etanercept protected from endotoxin lethality. In cultured bone marrow cells derived from the Tg mice, overexpressed IL-32α accelerated production of TNFα upon stimulation with LPS. Of note, exogenously added IL-32α alone stimulated RAW264.7 cells to express TNFα, IL-6, and MIP-2 mRNAs. Particularly, IL-32α -induced TNFα, but not IL-6 or MIP-2, was inhibited by dehydroxymethylepoxyquinomicin (DHMEQ) and U0126, which are specific inhibitors of nuclear factor kappa B (NFκB) and extracellular signal regulated kinase1/2 (ERK1/2), respectively.Conclusions: These results show that IL-32α contributed to the development of inflammatory arthritis and endotoxin lethality. Stimulation of TLR signaling with LPS appeared indispensable for activating the IL-32α-TNFα axis in vivo. However, IL-32α alone induced TNFα production in RAW264.7 cells through phosphorylation of inhibitor kappa B (IκB) and ERK1/2 MAPK. Further studies on the potential involvement of IL-32α-TNFα axis will be beneficial in better understanding the pathology of autoimmune-related arthritis and infectious immunity.

Original languageEnglish
Article numberR120
JournalArthritis Research and Therapy
Volume14
Issue number3
DOIs
Publication statusPublished - 2012 May 21

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Interleukins
Endotoxins
Transgenic Mice
Arthritis
Lipopolysaccharides
Shock
Tumor Necrosis Factor-alpha
Chemokine CXCL2
Interleukin-6
Zymosan
NF-kappa B
Toll-Like Receptors
Galactosamine
Messenger RNA
Infectious Arthritis
Injections
Synovitis
Mitogen-Activated Protein Kinase 1
Synovial Fluid
Articular Cartilage

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Immunology and Allergy
  • Medicine(all)

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Enhanced susceptibility to lipopolysaccharide-induced arthritis and endotoxin shock in interleukin-32 alpha transgenic mice through induction of tumor necrosis factor alpha. / Nakayama, Masanori; Niki, Yasuo; Kawasaki, Toshiki; Takeda, Yuki; Horiuchi, Keisuke; Sasaki, Aya; Okada, Yasunori; Umezawa, Kazuo; Ikegami, Hiroyasu; Toyama, Yoshiaki; Miyamoto, Takeshi.

In: Arthritis Research and Therapy, Vol. 14, No. 3, R120, 21.05.2012.

Research output: Contribution to journalArticle

Nakayama, Masanori ; Niki, Yasuo ; Kawasaki, Toshiki ; Takeda, Yuki ; Horiuchi, Keisuke ; Sasaki, Aya ; Okada, Yasunori ; Umezawa, Kazuo ; Ikegami, Hiroyasu ; Toyama, Yoshiaki ; Miyamoto, Takeshi. / Enhanced susceptibility to lipopolysaccharide-induced arthritis and endotoxin shock in interleukin-32 alpha transgenic mice through induction of tumor necrosis factor alpha. In: Arthritis Research and Therapy. 2012 ; Vol. 14, No. 3.
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author = "Masanori Nakayama and Yasuo Niki and Toshiki Kawasaki and Yuki Takeda and Keisuke Horiuchi and Aya Sasaki and Yasunori Okada and Kazuo Umezawa and Hiroyasu Ikegami and Yoshiaki Toyama and Takeshi Miyamoto",
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AU - Takeda, Yuki

AU - Horiuchi, Keisuke

AU - Sasaki, Aya

AU - Okada, Yasunori

AU - Umezawa, Kazuo

AU - Ikegami, Hiroyasu

AU - Toyama, Yoshiaki

AU - Miyamoto, Takeshi

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N2 - Introduction: The present study assessed the potential functions of interleukin (IL)-32α on inflammatory arthritis and endotoxin shock models using IL-32α transgenic (Tg) mice. The potential signaling pathway for the IL-32-tumor necrosis factor (TNF)α axis was analyzed in vitro.Methods: IL-32α Tg mice were generated under control of a ubiquitous promoter. Two disease models were used to examine in vivo effects of overexpressed IL-32α: Toll-like receptor (TLR) ligand-induced arthritis developed using a single injection of lipopolysaccharide (LPS) or zymosan into the knee joints; and endotoxin shock induced with intraperitoneal injection of LPS and D-galactosamine. TNFα antagonist etanercept was administered simultaneously with LPS in some mice. Using RAW264.7 cells, in vitro effects of exogenous IL-32α on TNFα, IL-6 or macrophage inflammatory protein 2 (MIP-2) production were assessed with or without inhibitors for nuclear factor kappa B (NFκB) or mitogen-activated protein kinase (MAPK).Results: Single injection of LPS, but not zymosan, resulted in development of severe synovitis with substantial articular cartilage degradation in knees of the Tg mice. The expression of TNFα mRNA in inflamed synovia was highly upregulated in the LPS-injected Tg mice. Moreover, the Tg mice were more susceptive to endotoxin-induced lethality than the wild-type control mice 48 hours after LPS challenge; but blockade of TNFα by etanercept protected from endotoxin lethality. In cultured bone marrow cells derived from the Tg mice, overexpressed IL-32α accelerated production of TNFα upon stimulation with LPS. Of note, exogenously added IL-32α alone stimulated RAW264.7 cells to express TNFα, IL-6, and MIP-2 mRNAs. Particularly, IL-32α -induced TNFα, but not IL-6 or MIP-2, was inhibited by dehydroxymethylepoxyquinomicin (DHMEQ) and U0126, which are specific inhibitors of nuclear factor kappa B (NFκB) and extracellular signal regulated kinase1/2 (ERK1/2), respectively.Conclusions: These results show that IL-32α contributed to the development of inflammatory arthritis and endotoxin lethality. Stimulation of TLR signaling with LPS appeared indispensable for activating the IL-32α-TNFα axis in vivo. However, IL-32α alone induced TNFα production in RAW264.7 cells through phosphorylation of inhibitor kappa B (IκB) and ERK1/2 MAPK. Further studies on the potential involvement of IL-32α-TNFα axis will be beneficial in better understanding the pathology of autoimmune-related arthritis and infectious immunity.

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