Enhanced transfection efficiency in laser-induced stress wave-assisted gene transfer at low laser fluence by increasing pressure impulse

Shinta Takano, Shunichi Sato, Mitsuhiro Terakawa, Hiroshi Asida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

To improve transfection efficiency in gene delivery based on nanosecond pulsed laser-Induced stress waves, we examined different types of transparent materials, a polyethylene terephthalate) sheet, polyvinyl alcohol) gel, and water, which were placed on a laser target for plasma confinement. We found that the use of water was most effective for maintaining a large pressure impulse during multipulse laser irradiation and, as a result, high transfection efficiency was demonstrated in rat skin in vivo at a relatively low laser fluence of 0.7 J/cm2. At this fluence, steady laser transmission through quartz fibers was confirmed, allowing endoscopic application of our gene delivery technique.

Original languageEnglish
Pages (from-to)380011-380013
Number of pages3
JournalApplied Physics Express
Volume1
Issue number3
DOIs
Publication statusPublished - 2008 Mar 1

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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