Enhanced transfection efficiency in laser-induced stress wave-assisted gene transfer at low laser fluence by increasing pressure impulse

Shinta Takano, Shunichi Sato, Mitsuhiro Terakawa, Hiroshi Asida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To improve transfection efficiency in gene delivery based on nanosecond pulsed laser-Induced stress waves, we examined different types of transparent materials, a polyethylene terephthalate) sheet, polyvinyl alcohol) gel, and water, which were placed on a laser target for plasma confinement. We found that the use of water was most effective for maintaining a large pressure impulse during multipulse laser irradiation and, as a result, high transfection efficiency was demonstrated in rat skin in vivo at a relatively low laser fluence of 0.7 J/cm2. At this fluence, steady laser transmission through quartz fibers was confirmed, allowing endoscopic application of our gene delivery technique.

Original languageEnglish
Pages (from-to)380011-380013
Number of pages3
JournalApplied Physics Express
Volume1
Issue number3
DOIs
Publication statusPublished - 2008 Mar

Fingerprint

Gene transfer
stress waves
genes
impulses
fluence
Lasers
delivery
Genes
lasers
laser targets
Plasma confinement
transparence
plasma control
polyvinyl alcohol
Polyvinyl alcohols
polyethylene terephthalate
Laser beam effects
Pulsed lasers
Polyethylene terephthalates
water

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Enhanced transfection efficiency in laser-induced stress wave-assisted gene transfer at low laser fluence by increasing pressure impulse. / Takano, Shinta; Sato, Shunichi; Terakawa, Mitsuhiro; Asida, Hiroshi; Okano, Hideyuki; Obara, Minoru.

In: Applied Physics Express, Vol. 1, No. 3, 03.2008, p. 380011-380013.

Research output: Contribution to journalArticle

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