Enhancement of Antitumor Activity of Cisplatin on Human Gastric Cancer Cells in vitro and in vivo by Buthionine Sulfoximine

Yoshiro Saikawa, Tetsuro Kubota, Tsong‐Hong ‐H Kuo, Toshiharu Furukawa, Hirokazu Tanino, Masahiko Watanabe, Kyuya Ishibiki, Masaki Kitajima

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An attempt was made to evaluate the enhancement of the antitumor activity of cisplatin (DDP) by buthionine sulfoximine (BSO) in vitro and in vivo. In the in vitro study, pre‐treatment with BSO (5, 10 and 25 mM) increased the antitumor activity of DDP against the gastric cancer cell lines MKN‐28 and MKN‐45, whereas BSO alone exhibited only slight antitumor activity (inhibition rate, 20–30%). In the in vivo study, the antitumor effects of DDP against human gastric cancer xenografts St‐15 and SC‐l‐NU in BALB/c nu/nu mice were enhanced pretreatment with BSO, which was administered intraperitoneally at a dose of 500 mg/kg according to a schedule of qd × 3. BSO alone showed no antitumor effects against these tumors in nude mice. The side effects (assessed in terms of death rate and body weight loss) associated with the maximum tolerated dose of DDP (9 mg/kg) were not increased by BSO pretreatment. As BSO increased the antitumor activity of DDP without a corresponding increment of its toxicity, BSO appears to be a promising agent for further study.

Original languageEnglish
Pages (from-to)787-793
Number of pages7
JournalJapanese Journal of Cancer Research
Volume84
Issue number7
DOIs
Publication statusPublished - 1993 Jul

Keywords

  • Buthionine sulfoximine
  • Cisplatin
  • Human gastric cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Enhancement of Antitumor Activity of Cisplatin on Human Gastric Cancer Cells in vitro and in vivo by Buthionine Sulfoximine'. Together they form a unique fingerprint.

  • Cite this