Enhancement of ectopic bone formation by bone morphogenetic protein-2 delivery using heparin-conjugated PLGA nanoparticles with transplantation of bone marrow-derived mesenchymal stem cells

Sung Eun Kim, Oju Jeon, Jung Bok Lee, Min Soo Bae, Heoung Jae Chun, Seong Hwan Moon, Il Keun Kwon

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

This study was performed to determine if a combination of previously undifferentiated bone marrow-derived mesenchymal stem cells (BMMSCs) and exogenous bone morphogenetic protein-2 (BMP-2) delivered via heparin-conjugated PLGA nanoparticles (HCPNs) would extensively regenerate bone in vivo. In vitro testing found that the HCPNs were able to release BMP-2 over a 2-week period. Human BMMSCs cultured in medium containing BMP-2-loaded HCPNs for 2 weeks differentiated toward osteogenic cells expressing alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) mRNA, while cells without BMP-2 expressed only ALP. In vivo testing found that undifferentiated BMMSCs with BMP-2-loaded HCPNs induce far more extensive bone formation than either implantation of BMP-2-loaded HCPNs or osteogenically differentiated BMMSCs. This study demonstrates the feasibility of extensive in vivo bone regeneration by transplantation of undifferentiated BMMSCs and BMP-2 delivery via HCPNs.

Original languageEnglish
Pages (from-to)771-777
Number of pages7
JournalJournal of Biomedical Science
Volume15
Issue number6
DOIs
Publication statusPublished - 2008 Nov 1
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 2
Stem cells
Bone Marrow Transplantation
Mesenchymal Stromal Cells
Osteogenesis
Nanoparticles
Heparin
Bone
Bone and Bones
Bone Marrow
Alkaline Phosphatase
Osteopontin
Bone Regeneration
Bone Transplantation
Osteocalcin
Feasibility Studies
polylactic acid-polyglycolic acid copolymer
Testing
Cells
Messenger RNA

Keywords

  • Bone marrow-derived mesenchymal stem cell
  • Bone morphogenetic protein-2
  • Bone regeneration
  • Heparin-conjugated PLGA nanoparticles

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Enhancement of ectopic bone formation by bone morphogenetic protein-2 delivery using heparin-conjugated PLGA nanoparticles with transplantation of bone marrow-derived mesenchymal stem cells. / Kim, Sung Eun; Jeon, Oju; Lee, Jung Bok; Bae, Min Soo; Chun, Heoung Jae; Moon, Seong Hwan; Kwon, Il Keun.

In: Journal of Biomedical Science, Vol. 15, No. 6, 01.11.2008, p. 771-777.

Research output: Contribution to journalArticle

Kim, Sung Eun ; Jeon, Oju ; Lee, Jung Bok ; Bae, Min Soo ; Chun, Heoung Jae ; Moon, Seong Hwan ; Kwon, Il Keun. / Enhancement of ectopic bone formation by bone morphogenetic protein-2 delivery using heparin-conjugated PLGA nanoparticles with transplantation of bone marrow-derived mesenchymal stem cells. In: Journal of Biomedical Science. 2008 ; Vol. 15, No. 6. pp. 771-777.
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