Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination

Keisuke Isobe, Takanori Takeda, Kyohei Mochizuki, Qiyuan Song, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, Katsumi Midorikawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We demonstrate super-resolution imaging with background fluorescence rejection by interferometric temporal focusing microscopy, in which temporal focusing is combined with structured illumination. The lateral resolution and the optical sectioning capability are simultaneously improved by factors of 1.6 and 1.4, respectively, compared to conventional temporal focusing microscopy. Fluorescent beads (200 nm diameter) that are difficult to distinguish from the background fluorescence in conventional temporal focusing microscopy, are clearly visualized by interferometric temporal focusing microscopy.

Original languageEnglish
Pages (from-to)2396-2410
Number of pages15
JournalBiomedical Optics Express
Volume4
Issue number11
DOIs
Publication statusPublished - 2013 Nov 1

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Lighting
Photons
Fluorescence Microscopy
illumination
Microscopy
microscopy
fluorescence
augmentation
photons
Fluorescence
beads
rejection

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination. / Isobe, Keisuke; Takeda, Takanori; Mochizuki, Kyohei; Song, Qiyuan; Suda, Akira; Kannari, Fumihiko; Kawano, Hiroyuki; Kumagai, Akiko; Miyawaki, Atsushi; Midorikawa, Katsumi.

In: Biomedical Optics Express, Vol. 4, No. 11, 01.11.2013, p. 2396-2410.

Research output: Contribution to journalArticle

Isobe, Keisuke ; Takeda, Takanori ; Mochizuki, Kyohei ; Song, Qiyuan ; Suda, Akira ; Kannari, Fumihiko ; Kawano, Hiroyuki ; Kumagai, Akiko ; Miyawaki, Atsushi ; Midorikawa, Katsumi. / Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination. In: Biomedical Optics Express. 2013 ; Vol. 4, No. 11. pp. 2396-2410.
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