Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination

Keisuke Isobe, Takanori Takeda, Kyohei Mochizuki, Qiyuan Song, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, Katsumi Midorikawa

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    We demonstrate super-resolution imaging with background fluorescence rejection by interferometric temporal focusing microscopy, in which temporal focusing is combined with structured illumination. The lateral resolution and the optical sectioning capability are simultaneously improved by factors of 1.6 and 1.4, respectively, compared to conventional temporal focusing microscopy. Fluorescent beads (200 nm diameter) that are difficult to distinguish from the background fluorescence in conventional temporal focusing microscopy, are clearly visualized by interferometric temporal focusing microscopy.

    Original languageEnglish
    Pages (from-to)2396-2410
    Number of pages15
    JournalBiomedical Optics Express
    Volume4
    Issue number11
    DOIs
    Publication statusPublished - 2013 Nov 1

    ASJC Scopus subject areas

    • Biotechnology
    • Atomic and Molecular Physics, and Optics

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