Enhancement of optical sectioning capability of temporal focusing microscopy by using time-multiplexed multi-line focusing

Kenta Inazawa, Keisuke Isobe, Tomohiro Ishikawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Research output: Contribution to journalArticlepeer-review

Abstract

Temporal focusing (TF) microscopy has a trade-off between temporal resolution, optical sectioning capability, and field of view. To improve this trade-off, we demonstrate time-multiplexed multi-line temporal focusing (TM-ML-TF) microscopy, which combines the time-multiplexed TF technique with multi-line focusing using a digital micromirror device. Compared with those for TF microscopy, the optical sectioning capability and the signal-to-background ratio were improved by factors of 2.5 and 4.17, respectively. In addition, by applying post-processing for structured illumination microscopy to TM-ML-TF microscopy, the optical sectioning capability and the signal-to-background ratio were further enhanced by factors of 1.2 and 4.63, respectively.

Original languageEnglish
Article number082008
JournalApplied Physics Express
Volume14
Issue number8
DOIs
Publication statusPublished - 2021 Aug

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint

Dive into the research topics of 'Enhancement of optical sectioning capability of temporal focusing microscopy by using time-multiplexed multi-line focusing'. Together they form a unique fingerprint.

Cite this