Enhancement of sparc (Osteonectin) synthesis in arthritic cartilage

Increased levels in synovial fluids from patients with rheumatoid arthritis and regulation by growth factors and cytokines in chondrocyte cultures

Shigeo Nakamura, Kyoko Kamihagi, Hisashi Satakeda, Masahiko Katayama, Haiou Pan, Hiroshi Okamoto, Mitsuhide Noshiro, Koichiro Takahashi, Yasuo Yoshihara, Masayuki Shimmei, Yasunori Okada, Yukio Kato

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Objective. To investigate the roles of SPARC (secreted protein, acidic and rich in cysteine) (osteonectin) in arthritis, using cartilage and synovium specimens and synovial fluids (SF) from patients with rheumatoid arthritis (RA) or osteoarthritis (OA), and to examine the effects of cytokines, growth factors, and hormones on SPARC synthesis by chondrocytes in culture. Methods. SPARC in cartilage and synovium was immunostained with monoclonal antibodies. SPARC synthesis by cultured chondrocytes was measured by Northern blot analysis, immunoblotting, and sandwich enzyme-linked immunosorbent assay. Results. SPARC was identified in numerous chondrocytes in the superficial and middle zones and in regenerating chondrocytes of RA and OA joints, whereas such staining was absent in these zones of normal cartilage, except for weak signals from a few chondrocytes in the deep zone. In addition, SPARC synthesis was enhanced in synovial cells of RA and OA joints. The average SPARC level in SF was 10-fold higher in the RA than in the OA population. In rabbit articular chondrocyte cultures, administration of transforming growth factor β1 (TGFβ1) and bone morphogenetic protein 2 increased SPARC levels at 24-48 hours, whereas interleukin-1β (IL-1β), IL-1α, tumor necrosis factor α, lipopolysaccharide, phorbol myristate acetate, basic fibroblast growth factor, and dexamethasone decreased SPARC levels at 24-72 hours. TGFβ increased SPARC messenger RNA (mRNA) levels at 24 hours, whereas IL-1β caused a marked decrease in SPARC mRNA levels at 24 hours. Furthermore, IL-1 decreased the glycosylation of SPARC. Conclusion. These findings suggest that various growth factors and cytokines, including TGFβ1 and IL-1β, regulate the production of SPARC by chondrocytes at pre- and posttranslational levels, and that SPARC synthesis is markedly enhanced in arthritic joints.

Original languageEnglish
Pages (from-to)539-551
Number of pages13
JournalArthritis and Rheumatism
Volume39
Issue number4
Publication statusPublished - 1996 Apr

Fingerprint

Osteonectin
Synovial Fluid
Chondrocytes
Arthritis
Cartilage
Cysteine
Rheumatoid Arthritis
Intercellular Signaling Peptides and Proteins
Cytokines
Proteins
Interleukin-1
Osteoarthritis
Joints
Synovial Membrane
Transforming Growth Factors
Bone Morphogenetic Protein 2
Messenger RNA
Tetradecanoylphorbol Acetate
Fibroblast Growth Factor 2

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Enhancement of sparc (Osteonectin) synthesis in arthritic cartilage : Increased levels in synovial fluids from patients with rheumatoid arthritis and regulation by growth factors and cytokines in chondrocyte cultures. / Nakamura, Shigeo; Kamihagi, Kyoko; Satakeda, Hisashi; Katayama, Masahiko; Pan, Haiou; Okamoto, Hiroshi; Noshiro, Mitsuhide; Takahashi, Koichiro; Yoshihara, Yasuo; Shimmei, Masayuki; Okada, Yasunori; Kato, Yukio.

In: Arthritis and Rheumatism, Vol. 39, No. 4, 04.1996, p. 539-551.

Research output: Contribution to journalArticle

Nakamura, S, Kamihagi, K, Satakeda, H, Katayama, M, Pan, H, Okamoto, H, Noshiro, M, Takahashi, K, Yoshihara, Y, Shimmei, M, Okada, Y & Kato, Y 1996, 'Enhancement of sparc (Osteonectin) synthesis in arthritic cartilage: Increased levels in synovial fluids from patients with rheumatoid arthritis and regulation by growth factors and cytokines in chondrocyte cultures', Arthritis and Rheumatism, vol. 39, no. 4, pp. 539-551.
Nakamura, Shigeo ; Kamihagi, Kyoko ; Satakeda, Hisashi ; Katayama, Masahiko ; Pan, Haiou ; Okamoto, Hiroshi ; Noshiro, Mitsuhide ; Takahashi, Koichiro ; Yoshihara, Yasuo ; Shimmei, Masayuki ; Okada, Yasunori ; Kato, Yukio. / Enhancement of sparc (Osteonectin) synthesis in arthritic cartilage : Increased levels in synovial fluids from patients with rheumatoid arthritis and regulation by growth factors and cytokines in chondrocyte cultures. In: Arthritis and Rheumatism. 1996 ; Vol. 39, No. 4. pp. 539-551.
@article{f64a2d0730ef4e77a4ad8ccfa82cbbee,
title = "Enhancement of sparc (Osteonectin) synthesis in arthritic cartilage: Increased levels in synovial fluids from patients with rheumatoid arthritis and regulation by growth factors and cytokines in chondrocyte cultures",
abstract = "Objective. To investigate the roles of SPARC (secreted protein, acidic and rich in cysteine) (osteonectin) in arthritis, using cartilage and synovium specimens and synovial fluids (SF) from patients with rheumatoid arthritis (RA) or osteoarthritis (OA), and to examine the effects of cytokines, growth factors, and hormones on SPARC synthesis by chondrocytes in culture. Methods. SPARC in cartilage and synovium was immunostained with monoclonal antibodies. SPARC synthesis by cultured chondrocytes was measured by Northern blot analysis, immunoblotting, and sandwich enzyme-linked immunosorbent assay. Results. SPARC was identified in numerous chondrocytes in the superficial and middle zones and in regenerating chondrocytes of RA and OA joints, whereas such staining was absent in these zones of normal cartilage, except for weak signals from a few chondrocytes in the deep zone. In addition, SPARC synthesis was enhanced in synovial cells of RA and OA joints. The average SPARC level in SF was 10-fold higher in the RA than in the OA population. In rabbit articular chondrocyte cultures, administration of transforming growth factor β1 (TGFβ1) and bone morphogenetic protein 2 increased SPARC levels at 24-48 hours, whereas interleukin-1β (IL-1β), IL-1α, tumor necrosis factor α, lipopolysaccharide, phorbol myristate acetate, basic fibroblast growth factor, and dexamethasone decreased SPARC levels at 24-72 hours. TGFβ increased SPARC messenger RNA (mRNA) levels at 24 hours, whereas IL-1β caused a marked decrease in SPARC mRNA levels at 24 hours. Furthermore, IL-1 decreased the glycosylation of SPARC. Conclusion. These findings suggest that various growth factors and cytokines, including TGFβ1 and IL-1β, regulate the production of SPARC by chondrocytes at pre- and posttranslational levels, and that SPARC synthesis is markedly enhanced in arthritic joints.",
author = "Shigeo Nakamura and Kyoko Kamihagi and Hisashi Satakeda and Masahiko Katayama and Haiou Pan and Hiroshi Okamoto and Mitsuhide Noshiro and Koichiro Takahashi and Yasuo Yoshihara and Masayuki Shimmei and Yasunori Okada and Yukio Kato",
year = "1996",
month = "4",
language = "English",
volume = "39",
pages = "539--551",
journal = "Arthritis and Rheumatology",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - Enhancement of sparc (Osteonectin) synthesis in arthritic cartilage

T2 - Increased levels in synovial fluids from patients with rheumatoid arthritis and regulation by growth factors and cytokines in chondrocyte cultures

AU - Nakamura, Shigeo

AU - Kamihagi, Kyoko

AU - Satakeda, Hisashi

AU - Katayama, Masahiko

AU - Pan, Haiou

AU - Okamoto, Hiroshi

AU - Noshiro, Mitsuhide

AU - Takahashi, Koichiro

AU - Yoshihara, Yasuo

AU - Shimmei, Masayuki

AU - Okada, Yasunori

AU - Kato, Yukio

PY - 1996/4

Y1 - 1996/4

N2 - Objective. To investigate the roles of SPARC (secreted protein, acidic and rich in cysteine) (osteonectin) in arthritis, using cartilage and synovium specimens and synovial fluids (SF) from patients with rheumatoid arthritis (RA) or osteoarthritis (OA), and to examine the effects of cytokines, growth factors, and hormones on SPARC synthesis by chondrocytes in culture. Methods. SPARC in cartilage and synovium was immunostained with monoclonal antibodies. SPARC synthesis by cultured chondrocytes was measured by Northern blot analysis, immunoblotting, and sandwich enzyme-linked immunosorbent assay. Results. SPARC was identified in numerous chondrocytes in the superficial and middle zones and in regenerating chondrocytes of RA and OA joints, whereas such staining was absent in these zones of normal cartilage, except for weak signals from a few chondrocytes in the deep zone. In addition, SPARC synthesis was enhanced in synovial cells of RA and OA joints. The average SPARC level in SF was 10-fold higher in the RA than in the OA population. In rabbit articular chondrocyte cultures, administration of transforming growth factor β1 (TGFβ1) and bone morphogenetic protein 2 increased SPARC levels at 24-48 hours, whereas interleukin-1β (IL-1β), IL-1α, tumor necrosis factor α, lipopolysaccharide, phorbol myristate acetate, basic fibroblast growth factor, and dexamethasone decreased SPARC levels at 24-72 hours. TGFβ increased SPARC messenger RNA (mRNA) levels at 24 hours, whereas IL-1β caused a marked decrease in SPARC mRNA levels at 24 hours. Furthermore, IL-1 decreased the glycosylation of SPARC. Conclusion. These findings suggest that various growth factors and cytokines, including TGFβ1 and IL-1β, regulate the production of SPARC by chondrocytes at pre- and posttranslational levels, and that SPARC synthesis is markedly enhanced in arthritic joints.

AB - Objective. To investigate the roles of SPARC (secreted protein, acidic and rich in cysteine) (osteonectin) in arthritis, using cartilage and synovium specimens and synovial fluids (SF) from patients with rheumatoid arthritis (RA) or osteoarthritis (OA), and to examine the effects of cytokines, growth factors, and hormones on SPARC synthesis by chondrocytes in culture. Methods. SPARC in cartilage and synovium was immunostained with monoclonal antibodies. SPARC synthesis by cultured chondrocytes was measured by Northern blot analysis, immunoblotting, and sandwich enzyme-linked immunosorbent assay. Results. SPARC was identified in numerous chondrocytes in the superficial and middle zones and in regenerating chondrocytes of RA and OA joints, whereas such staining was absent in these zones of normal cartilage, except for weak signals from a few chondrocytes in the deep zone. In addition, SPARC synthesis was enhanced in synovial cells of RA and OA joints. The average SPARC level in SF was 10-fold higher in the RA than in the OA population. In rabbit articular chondrocyte cultures, administration of transforming growth factor β1 (TGFβ1) and bone morphogenetic protein 2 increased SPARC levels at 24-48 hours, whereas interleukin-1β (IL-1β), IL-1α, tumor necrosis factor α, lipopolysaccharide, phorbol myristate acetate, basic fibroblast growth factor, and dexamethasone decreased SPARC levels at 24-72 hours. TGFβ increased SPARC messenger RNA (mRNA) levels at 24 hours, whereas IL-1β caused a marked decrease in SPARC mRNA levels at 24 hours. Furthermore, IL-1 decreased the glycosylation of SPARC. Conclusion. These findings suggest that various growth factors and cytokines, including TGFβ1 and IL-1β, regulate the production of SPARC by chondrocytes at pre- and posttranslational levels, and that SPARC synthesis is markedly enhanced in arthritic joints.

UR - http://www.scopus.com/inward/record.url?scp=0029923070&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029923070&partnerID=8YFLogxK

M3 - Article

VL - 39

SP - 539

EP - 551

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 4

ER -