Enhancing effect of the liver extract and flavin adenin dinucleotide mixture on anti-viral efficacy of interferon in patients with chronic hepatitis C

Hidetsugu Saito, Hirotoshi Ebinuma, Shinichiro Tada, Satoshi Tsunematsu, Kazuhiro Atsukawa, Tetsuya Masuda, Masaharu Tsuchiya, Hiromasa Ishii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A combined preparation of liver extract and flavin adenin dinucleotide (FAD) (Adelavin®) has been widely used in patients with chronic liver diseases in Japan. One milliliter of this agent contains 15 μl of phenol-soluble phase of liver nucleic acid fraction and 10 mg of FAD. To examine the advantages of using this preparation in the elimination of hepatitis C virus (HCV) from patients with chronic hepatitis (CH)-C receiving interferon (IFN), 2 ml of this preparation was intravenously (n = 9) or intramuscularly (n = 8) administered daily for 5 days before 6 million units of IFN-α was intramuscularly injected once. Before and 48 hours after the injection of IFN, serum ALT, 2'5'-oligoadenylate synthetase (2'5-AS) activity, and HCV RNA levels were measured. The daily administration of this preparation alone for 5 days did not significantly change serum ALT, 2'5-AS activities, and HCV RNA levels. The 2'5-AS activities were significantly increased by IFN after the intravenous injection of this preparation (p < 0.01), while an injection of IFN alone of this dose did not change its activities (n = 10). HCV RNA levels were significantly decreased by IFN only after the administration of the preparation (intramuscular, p < 0.01; intravenous, p < 0.01). The effect of intravenous injection of this preparation was also elicited in patients with HCV genotype D and with HCV more than 105 copies/ml. These results suggest that this preparation may enhance the 2'5-AS production by IFN as a result of the increase in mitochondrial adenosin triphosphate production and may be a potent agent to enhance the anti-viral efficacy of IFN in patients with CH-C.

Original languageEnglish
Pages (from-to)48-53
Number of pages6
JournalKeio Journal of Medicine
Volume45
Issue number1
DOIs
Publication statusPublished - 1996 Mar

Keywords

  • chronic hepatitis C
  • flavin adenin dinucleotide
  • interferon
  • liver extract

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Enhancing effect of the liver extract and flavin adenin dinucleotide mixture on anti-viral efficacy of interferon in patients with chronic hepatitis C'. Together they form a unique fingerprint.

  • Cite this