Abstract
Four isolates of Entamoeba dispar identified by their hexokinase and phosphoglucomutase isoenzyme profile and by their failure to react with Entamoeba histolytica-specific monoclonal antibody (4G6) could be grown in either Diamond's BI-S-33 medium, newly developed BCSI-S (Biosate cysteine starch iron-serum) medium, or casein-free YI-S medium in the presence of Crithidia fasciculata (ReF-1:PRR) sterilized by heating 56°C for 30 min and subsequent incubation with 1% hydrogen peroxide for 24 hours at 4°C. After the cultures were maintained for over 50 passages, the amebae were identified as E. dispar by isoenzyme analysis, polymerase chain reaction with E. histolytica- and E. dispar-specific primers, i.e. p11 plus p12 and p13 plus p14, respectively, and by negative reactivity with monoclonal antibody 4G6. The flagellates added to the culture were judged to be metabolically inactive based on the results of nuclear magnetic resonance spectroscopy, electron microscopy, and polarographic analysis. All of these findings suggest that E. dispar can grow in vitro with metabolically inactive C. fasciculata as a culture associate.
| Original language | English |
|---|---|
| Journal | Journal of Eukaryotic Microbiology |
| Volume | 45 |
| Issue number | 2 |
| Publication status | Published - 1998 Mar |
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Keywords
- Crithidia fasciculata
- Entamoeba dispar
- Hydrogen peroxide
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Applied Microbiology and Biotechnology
- Agricultural and Biological Sciences (miscellaneous)
- Microbiology
Cite this
Entamoeba dispar : Cultivation with sterilized Crithidia fasciculata. / Kobayashi, Seiki; Imai, Eiko; Tachibana, Hiroshi; Fujiwara, Tatsushi; Takeuchi, Tsutomu.
In: Journal of Eukaryotic Microbiology, Vol. 45, No. 2, 03.1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Entamoeba dispar
T2 - Cultivation with sterilized Crithidia fasciculata
AU - Kobayashi, Seiki
AU - Imai, Eiko
AU - Tachibana, Hiroshi
AU - Fujiwara, Tatsushi
AU - Takeuchi, Tsutomu
PY - 1998/3
Y1 - 1998/3
N2 - Four isolates of Entamoeba dispar identified by their hexokinase and phosphoglucomutase isoenzyme profile and by their failure to react with Entamoeba histolytica-specific monoclonal antibody (4G6) could be grown in either Diamond's BI-S-33 medium, newly developed BCSI-S (Biosate cysteine starch iron-serum) medium, or casein-free YI-S medium in the presence of Crithidia fasciculata (ReF-1:PRR) sterilized by heating 56°C for 30 min and subsequent incubation with 1% hydrogen peroxide for 24 hours at 4°C. After the cultures were maintained for over 50 passages, the amebae were identified as E. dispar by isoenzyme analysis, polymerase chain reaction with E. histolytica- and E. dispar-specific primers, i.e. p11 plus p12 and p13 plus p14, respectively, and by negative reactivity with monoclonal antibody 4G6. The flagellates added to the culture were judged to be metabolically inactive based on the results of nuclear magnetic resonance spectroscopy, electron microscopy, and polarographic analysis. All of these findings suggest that E. dispar can grow in vitro with metabolically inactive C. fasciculata as a culture associate.
AB - Four isolates of Entamoeba dispar identified by their hexokinase and phosphoglucomutase isoenzyme profile and by their failure to react with Entamoeba histolytica-specific monoclonal antibody (4G6) could be grown in either Diamond's BI-S-33 medium, newly developed BCSI-S (Biosate cysteine starch iron-serum) medium, or casein-free YI-S medium in the presence of Crithidia fasciculata (ReF-1:PRR) sterilized by heating 56°C for 30 min and subsequent incubation with 1% hydrogen peroxide for 24 hours at 4°C. After the cultures were maintained for over 50 passages, the amebae were identified as E. dispar by isoenzyme analysis, polymerase chain reaction with E. histolytica- and E. dispar-specific primers, i.e. p11 plus p12 and p13 plus p14, respectively, and by negative reactivity with monoclonal antibody 4G6. The flagellates added to the culture were judged to be metabolically inactive based on the results of nuclear magnetic resonance spectroscopy, electron microscopy, and polarographic analysis. All of these findings suggest that E. dispar can grow in vitro with metabolically inactive C. fasciculata as a culture associate.
KW - Crithidia fasciculata
KW - Entamoeba dispar
KW - Hydrogen peroxide
UR - http://www.scopus.com/inward/record.url?scp=0032033621&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032033621&partnerID=8YFLogxK
M3 - Article
C2 - 9561776
AN - SCOPUS:0032033621
VL - 45
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
SN - 1066-5234
IS - 2
ER -