Entamoeba dispar: Molecular characterization of the galactose/N-acetyl-D-galactosamine lectin

Dylan R. Pillai, Seiki Kobayashi, Kevin C. Kain

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Amebiasis contributes to approximately 50 million cases of life-threatening dysentery world-wide. Comparison of the lectins from Entamoeba histolytica (pathogenic) and Entamoeba dispar (nonpathogenic) was undertaken to elucidate the differential roles of this molecule in invasion versus colonization. Surface lectin was less abundant on axenic E. dispar than on axenic E. histolytica, commensurate with differences in lectin (heavy and light subunits) RNA when assessed by semiquantitative RT-PCR. The 1G7 epitope, which falls within the immunodominant and immuno-protective cysteine-rich region (480-900), was absent on axenic E. dispar. Indirect immunofluorescence, transient transection of COS7, and immunoprecipitation demonstrated that the 1G7 epitope was conserved in the nonpathogenic lectin homologue but not exposed on live E. dispar trophozoites. Hg12 (E. histolytica) and Dhg12 (E. dispar) lectin homologues demonstrated comparable high-affinity binding to multivalent GalNAc19BSA. These data provide evidence for relative gene and conformational regulation of the E. dispar lectin.

Original languageEnglish
Pages (from-to)226-234
Number of pages9
JournalExperimental Parasitology
Volume99
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Entamoeba
Acetylgalactosamine
Galactose
Lectins
Entamoeba histolytica
Epitopes
Amebiasis
Trophozoites
Dysentery
Indirect Fluorescent Antibody Technique
Immunoprecipitation
Cysteine
RNA
Light
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Entamoeba dispar : Molecular characterization of the galactose/N-acetyl-D-galactosamine lectin. / Pillai, Dylan R.; Kobayashi, Seiki; Kain, Kevin C.

In: Experimental Parasitology, Vol. 99, No. 4, 2001, p. 226-234.

Research output: Contribution to journalArticle

Pillai, Dylan R. ; Kobayashi, Seiki ; Kain, Kevin C. / Entamoeba dispar : Molecular characterization of the galactose/N-acetyl-D-galactosamine lectin. In: Experimental Parasitology. 2001 ; Vol. 99, No. 4. pp. 226-234.
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