TY - JOUR
T1 - Entamoeba histolytica
T2 - Differences in phagosome acidification and degradation between attenuated and virulent strains
AU - Mitra, Biswa Nath
AU - Kobayashi, Seiki
AU - Saito-Nakano, Yumiko
AU - Nozaki, Tomoyoshi
N1 - Funding Information:
The authors thank Shin-ichiro Kawazu, International Medical Center of Japan, Tokyo, and K.P. Chang, Department of Microbiology and Immunology, Finch University of Health Sciences, Chicago, for the GFP-expressing Leishmania amazonensis strain. We also thank Kumiko Nakada-Tsukui, Dan Sato, Mami Okada, Mai Nudejima for their technical assistance and helpful discussions. This work was supported in part by a grant for Precursory Research for Embryonic Science and Technology (PRESTO), the Japan Science and Technology Agency, a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to T.N. (15019120, 15590378, 16017307, and 16044250), a grant for Research on Emerging and Re-emerging Infectious Diseases from the Ministry of Health, Labour and Welfare, and a grant for the Project to Promote the Development of Anti-AIDS Pharmaceuticals from the Japan Health Sciences Foundation to T.N.
PY - 2006/9
Y1 - 2006/9
N2 - Phagocytosis is the important virulent determinant of the enteric protozoan parasite Entamoeba histolytica. We compared the kinetics of phagosome maturation of attenuated and highly-virulent strains of E. histolytica using video microscopy. Phagosomes of attenuated strains were acidified rapidly within 2 min after phagosome formation (at the rate of 0.96 pH/min), persisted at pH 4.46 ± 0.13, and degraded ingested GFP-Leishmania very efficiently (90-94% GFP fluorescence was lost in 30 min), while phagosomes of highly-virulent strains were acidified slowly (0.69 pH/min), persisted at 5.11 ± 0.23, and degraded GFP less efficiently (60-71% decrease). These results suggest that efficiency of phagosome maturation is most probably inversely correlated with apparent virulence.
AB - Phagocytosis is the important virulent determinant of the enteric protozoan parasite Entamoeba histolytica. We compared the kinetics of phagosome maturation of attenuated and highly-virulent strains of E. histolytica using video microscopy. Phagosomes of attenuated strains were acidified rapidly within 2 min after phagosome formation (at the rate of 0.96 pH/min), persisted at pH 4.46 ± 0.13, and degraded ingested GFP-Leishmania very efficiently (90-94% GFP fluorescence was lost in 30 min), while phagosomes of highly-virulent strains were acidified slowly (0.69 pH/min), persisted at 5.11 ± 0.23, and degraded GFP less efficiently (60-71% decrease). These results suggest that efficiency of phagosome maturation is most probably inversely correlated with apparent virulence.
KW - Attenuation
KW - Entamoeba histolytica
KW - Phagocytosis
KW - Vacuolar type ATPase
KW - Virulence
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UR - http://www.scopus.com/inward/citedby.url?scp=33746895778&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2006.02.009
DO - 10.1016/j.exppara.2006.02.009
M3 - Article
C2 - 16546173
AN - SCOPUS:33746895778
VL - 114
SP - 57
EP - 61
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 1
ER -
