Entamoeba histolytica: Differences in phagosome acidification and degradation between attenuated and virulent strains

Biswa Nath Mitra, Seiki Kobayashi, Yumiko Saito-Nakano, Tomoyoshi Nozaki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Phagocytosis is the important virulent determinant of the enteric protozoan parasite Entamoeba histolytica. We compared the kinetics of phagosome maturation of attenuated and highly-virulent strains of E. histolytica using video microscopy. Phagosomes of attenuated strains were acidified rapidly within 2 min after phagosome formation (at the rate of 0.96 pH/min), persisted at pH 4.46 ± 0.13, and degraded ingested GFP-Leishmania very efficiently (90-94% GFP fluorescence was lost in 30 min), while phagosomes of highly-virulent strains were acidified slowly (0.69 pH/min), persisted at 5.11 ± 0.23, and degraded GFP less efficiently (60-71% decrease). These results suggest that efficiency of phagosome maturation is most probably inversely correlated with apparent virulence.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalExperimental Parasitology
Volume114
Issue number1
DOIs
Publication statusPublished - 2006 Sep 1

Keywords

  • Attenuation
  • Entamoeba histolytica
  • Phagocytosis
  • Vacuolar type ATPase
  • Virulence

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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