TY - JOUR
T1 - Entamoeba invadens
T2 - Enhancement of excystation and metacystic development by cytochalasin D
AU - Makioka, Asao
AU - Kumagai, Masahiro
AU - Ohtomo, Hiroshi
AU - Kobayashi, Seiki
AU - Takeuchi, Tsutomu
N1 - Funding Information:
We thank Dr. L. S. Diamond for supplying the E. invadens and T. Okita for technical assistance. This work was supported by a Health Sciences Research Grant for Research on Emerging and Re-emerging Infectious Diseases from the Ministry of Health and Welfare of Japan.
PY - 2001
Y1 - 2001
N2 - Effects of three actin-modifying drugs, cytochalasin D, latrunculin A, and jasplakinolide, on the excystation and metacystic development in vitro of Entamoeba invadens were examined by transfer of the cysts to growth medium with the drugs. Cytochalasin D unexpectedly increased the number of metacystic amoebae of E. invadens strain IP-1 during incubation. Metacystic development, which was determined by the number of nuclei of metacystic amoebae, was faster in the culture with cytochalasin D than in the culture without the drug. These results suggest that cytochalasin D enhances the excystation and metacystic development. In contrast, latrunculin A and jasplakinolide inhibited these process. No excystation occurred in encystation medium even in the presence of cytochalasin D, suggesting that growth medium is essential for excystation. Excystation was further enhanced when the cysts were incubated with cytochalasin D before culture in growth medium with the drug. The enhancing effect of cytochalasin D on the excystation and metacystic development was abrogated by jasplakinolide. Thus, the results indicate that cytochalasin D, unlike latrunculin A and jasplakinolide, caused enhancement of the excystation and metacystic development of this parasite.
AB - Effects of three actin-modifying drugs, cytochalasin D, latrunculin A, and jasplakinolide, on the excystation and metacystic development in vitro of Entamoeba invadens were examined by transfer of the cysts to growth medium with the drugs. Cytochalasin D unexpectedly increased the number of metacystic amoebae of E. invadens strain IP-1 during incubation. Metacystic development, which was determined by the number of nuclei of metacystic amoebae, was faster in the culture with cytochalasin D than in the culture without the drug. These results suggest that cytochalasin D enhances the excystation and metacystic development. In contrast, latrunculin A and jasplakinolide inhibited these process. No excystation occurred in encystation medium even in the presence of cytochalasin D, suggesting that growth medium is essential for excystation. Excystation was further enhanced when the cysts were incubated with cytochalasin D before culture in growth medium with the drug. The enhancing effect of cytochalasin D on the excystation and metacystic development was abrogated by jasplakinolide. Thus, the results indicate that cytochalasin D, unlike latrunculin A and jasplakinolide, caused enhancement of the excystation and metacystic development of this parasite.
KW - Actin polymerization
KW - Cytochalasin D
KW - DMSO
KW - Dimethyl sulfoxide
KW - Entamoeba invadens
KW - Excystation
KW - Jasplakinolide
KW - Latrunculin A
KW - Metacystic development
KW - Protozoa
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U2 - 10.1006/expr.2001.4629
DO - 10.1006/expr.2001.4629
M3 - Article
C2 - 11527437
AN - SCOPUS:0034851989
SN - 0014-4894
VL - 98
SP - 145
EP - 151
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -
