Enzymatic inactivation of major circulating forms of atrial and brain natriuretic peptides

Junko Ozaki, Hiromasa Shimizu, Yukiya Hashimoto, Hiroshi Itoh, Kazuwa Nakao, Ken Ichi Inui

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We compared the enzymatic inactivation of major circulating forms of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP). Both ANP and BNP induced a significant increase in cyclic GMP (cGMP) formation in cultured epithelial cell line derived from porcine kidney, LLC-PK1. The cGMP formation stimulated by ANP in LLC-PK1 cells was significantly decreased by pre-treatment of the peptide with rat renal brush-border membranes, and the inactivation of ANP was inhibited by neutral endopeptidase inhibitors, phosphoramidon and S-thiorphan. BNP exhibited greater resistance to enzymatic inactivation than did ANP. In addition, phosphoramidon potentiated the natriuresis with a low dose (7.5 pmol min-1 kg-1) of ANP but not of BNP in rats. These results suggest that enzymatic degradation of natriuretic peptides is highly dependent on peptide structure, and that the affinity of BNP to neutral endopeptidase is less than that of ANP. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalEuropean Journal of Pharmacology
Volume370
Issue number3
DOIs
Publication statusPublished - 1999 Apr 16
Externally publishedYes

Fingerprint

Brain Natriuretic Peptide
Atrial Natriuretic Factor
Neprilysin
Cyclic GMP
Thiorphan
LLC-PK1 Cells
Kidney
Natriuretic Peptides
Natriuresis
Peptides
Microvilli
Protease Inhibitors
Cultured Cells
Swine
Epithelial Cells
Cell Line
Membranes

Keywords

  • ANP (Atrial natriuretic peptide)
  • BNP (Brain natriuretic peptide)
  • Kidney epithelial cell line (LLC-PK)
  • Neutral endopeptidase
  • Phosphoramidon
  • Thiorphan

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Enzymatic inactivation of major circulating forms of atrial and brain natriuretic peptides. / Ozaki, Junko; Shimizu, Hiromasa; Hashimoto, Yukiya; Itoh, Hiroshi; Nakao, Kazuwa; Inui, Ken Ichi.

In: European Journal of Pharmacology, Vol. 370, No. 3, 16.04.1999, p. 307-312.

Research output: Contribution to journalArticle

Ozaki, Junko ; Shimizu, Hiromasa ; Hashimoto, Yukiya ; Itoh, Hiroshi ; Nakao, Kazuwa ; Inui, Ken Ichi. / Enzymatic inactivation of major circulating forms of atrial and brain natriuretic peptides. In: European Journal of Pharmacology. 1999 ; Vol. 370, No. 3. pp. 307-312.
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