Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice

Yukako Tani, Yosuke Isobe, Yuki Imoto, Eri Segi-Nishida, Yukihiko Sugimoto, Hiroyuki Arai, Makoto Arita

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Resolution of inflammation is critical to restoration of tissue function after an inflammatory response. We previously demonstrated that 12/15-lipoxygenase (12/15-LOX)-expressing eosinophils contribute to this process in murine zymosan-induced peritonitis. In this study, eosinophils promoted resolution by regulating expression of macrophage CXCL13. Microarray analysis revealed that eosinophils significantly increased (∼3-fold) the expression of macrophage CXCL13 by a 12/15-LOX-dependent mechanism. CXCL13 depletion caused a resolution defect, with the reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph nodes. Inflamed lymph node hypertrophy, a critical feature of the resolution process, was reduced by ∼60% in eosinophil-deficient mice, and adoptive transfer of eosinophils or administration of CXCL13 corrected this defect. Administration of the 12/15-LOX-derived mediator lipoxin A4(LXA4) increased the expression of CXCL13 and restored the defect of lymph node hypertrophy in eosinophil-deficient mice. These results demonstrate that eosinophils control the resolution of inflammation and draining lymph node hypertrophy through proresolving lipid mediators and the CXCL13 pathway in mice.

Original languageEnglish
Pages (from-to)4036-4043
Number of pages8
JournalFASEB Journal
Volume28
Issue number9
DOIs
Publication statusPublished - 2014 Sep 1
Externally publishedYes

Fingerprint

Eosinophils
Hypertrophy
Zymosan
Macrophages
Lymph Nodes
Inflammation
Defects
Microarrays
Restoration
Tissue
Lipids
Adoptive Transfer
Microarray Analysis
Phagocytes
12-15-lipoxygenase
Peritonitis

Keywords

  • Lipid mediator
  • Lipoxygenase

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice. / Tani, Yukako; Isobe, Yosuke; Imoto, Yuki; Segi-Nishida, Eri; Sugimoto, Yukihiko; Arai, Hiroyuki; Arita, Makoto.

In: FASEB Journal, Vol. 28, No. 9, 01.09.2014, p. 4036-4043.

Research output: Contribution to journalArticle

Tani, Yukako ; Isobe, Yosuke ; Imoto, Yuki ; Segi-Nishida, Eri ; Sugimoto, Yukihiko ; Arai, Hiroyuki ; Arita, Makoto. / Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice. In: FASEB Journal. 2014 ; Vol. 28, No. 9. pp. 4036-4043.
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