Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice

Tomohiro Yamada, Yukako Tani, Hiroki Nakanishi, Ryo Taguchi, Makoto Arita, Hiroyuki Arai

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Acute inflammation in healthy individuals is self-limiting and has an active termination program. The mechanisms by which acute inflammation is resolved are of interest. In murine zymosan-induced peritonitis, we found that eosinophils are recruited to the inflamed loci during the resolution phase of acute inflammation. In vivo depletion of eosinophils caused a resolution deficit, namely impaired lymphatic drainage with reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph node, and sustained numbers of polymorphonuclear leukocytes in inflamed tissues. Liquid chromatography-tandem mass spectrometry-based lipidomics of the resolving exudates revealed that locally activated eosinophils in the resolution phase produced proresolving mediators, including protectin D1 (PD1) from docosa-hexaenoic acid. The resolution deficit caused by eosinophil depletion was rescued by eosinophil restoration or the administration of PD1. Eosinophils deficient in 12/15-lipoxygenase could not rescue the resolution phenotype. The present results indicate that mouse eosinophils and eosinophil-derived lipid mediators, including PD1, have a role in promoting the resolution of acute inflammation, expanding the roles of eosinophils in host defense and resolution.

Original languageEnglish
Pages (from-to)561-568
Number of pages8
JournalFASEB Journal
Volume25
Issue number2
DOIs
Publication statusPublished - 2011 Feb
Externally publishedYes

Fingerprint

Peritonitis
Eosinophils
Zymosan
Liquid chromatography
Drainage
Restoration
Inflammation
Mass spectrometry
Tissue
Lipids
Acids
protectin D1
Exudates and Transudates
Phagocytes
Tandem Mass Spectrometry
Liquid Chromatography
Neutrophils
Lymph Nodes
Phenotype

Keywords

  • Anti-inflammation
  • Lipid mediator
  • Lipoxygenase
  • Metabolomics
  • Protectin

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice. / Yamada, Tomohiro; Tani, Yukako; Nakanishi, Hiroki; Taguchi, Ryo; Arita, Makoto; Arai, Hiroyuki.

In: FASEB Journal, Vol. 25, No. 2, 02.2011, p. 561-568.

Research output: Contribution to journalArticle

Yamada, Tomohiro ; Tani, Yukako ; Nakanishi, Hiroki ; Taguchi, Ryo ; Arita, Makoto ; Arai, Hiroyuki. / Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice. In: FASEB Journal. 2011 ; Vol. 25, No. 2. pp. 561-568.
@article{b45ea4dfefe1481f85f2574486a4398c,
title = "Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice",
abstract = "Acute inflammation in healthy individuals is self-limiting and has an active termination program. The mechanisms by which acute inflammation is resolved are of interest. In murine zymosan-induced peritonitis, we found that eosinophils are recruited to the inflamed loci during the resolution phase of acute inflammation. In vivo depletion of eosinophils caused a resolution deficit, namely impaired lymphatic drainage with reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph node, and sustained numbers of polymorphonuclear leukocytes in inflamed tissues. Liquid chromatography-tandem mass spectrometry-based lipidomics of the resolving exudates revealed that locally activated eosinophils in the resolution phase produced proresolving mediators, including protectin D1 (PD1) from docosa-hexaenoic acid. The resolution deficit caused by eosinophil depletion was rescued by eosinophil restoration or the administration of PD1. Eosinophils deficient in 12/15-lipoxygenase could not rescue the resolution phenotype. The present results indicate that mouse eosinophils and eosinophil-derived lipid mediators, including PD1, have a role in promoting the resolution of acute inflammation, expanding the roles of eosinophils in host defense and resolution.",
keywords = "Anti-inflammation, Lipid mediator, Lipoxygenase, Metabolomics, Protectin",
author = "Tomohiro Yamada and Yukako Tani and Hiroki Nakanishi and Ryo Taguchi and Makoto Arita and Hiroyuki Arai",
year = "2011",
month = "2",
doi = "10.1096/fj.10-170027",
language = "English",
volume = "25",
pages = "561--568",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "2",

}

TY - JOUR

T1 - Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice

AU - Yamada, Tomohiro

AU - Tani, Yukako

AU - Nakanishi, Hiroki

AU - Taguchi, Ryo

AU - Arita, Makoto

AU - Arai, Hiroyuki

PY - 2011/2

Y1 - 2011/2

N2 - Acute inflammation in healthy individuals is self-limiting and has an active termination program. The mechanisms by which acute inflammation is resolved are of interest. In murine zymosan-induced peritonitis, we found that eosinophils are recruited to the inflamed loci during the resolution phase of acute inflammation. In vivo depletion of eosinophils caused a resolution deficit, namely impaired lymphatic drainage with reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph node, and sustained numbers of polymorphonuclear leukocytes in inflamed tissues. Liquid chromatography-tandem mass spectrometry-based lipidomics of the resolving exudates revealed that locally activated eosinophils in the resolution phase produced proresolving mediators, including protectin D1 (PD1) from docosa-hexaenoic acid. The resolution deficit caused by eosinophil depletion was rescued by eosinophil restoration or the administration of PD1. Eosinophils deficient in 12/15-lipoxygenase could not rescue the resolution phenotype. The present results indicate that mouse eosinophils and eosinophil-derived lipid mediators, including PD1, have a role in promoting the resolution of acute inflammation, expanding the roles of eosinophils in host defense and resolution.

AB - Acute inflammation in healthy individuals is self-limiting and has an active termination program. The mechanisms by which acute inflammation is resolved are of interest. In murine zymosan-induced peritonitis, we found that eosinophils are recruited to the inflamed loci during the resolution phase of acute inflammation. In vivo depletion of eosinophils caused a resolution deficit, namely impaired lymphatic drainage with reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph node, and sustained numbers of polymorphonuclear leukocytes in inflamed tissues. Liquid chromatography-tandem mass spectrometry-based lipidomics of the resolving exudates revealed that locally activated eosinophils in the resolution phase produced proresolving mediators, including protectin D1 (PD1) from docosa-hexaenoic acid. The resolution deficit caused by eosinophil depletion was rescued by eosinophil restoration or the administration of PD1. Eosinophils deficient in 12/15-lipoxygenase could not rescue the resolution phenotype. The present results indicate that mouse eosinophils and eosinophil-derived lipid mediators, including PD1, have a role in promoting the resolution of acute inflammation, expanding the roles of eosinophils in host defense and resolution.

KW - Anti-inflammation

KW - Lipid mediator

KW - Lipoxygenase

KW - Metabolomics

KW - Protectin

UR - http://www.scopus.com/inward/record.url?scp=79551616553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551616553&partnerID=8YFLogxK

U2 - 10.1096/fj.10-170027

DO - 10.1096/fj.10-170027

M3 - Article

C2 - 20959515

AN - SCOPUS:79551616553

VL - 25

SP - 561

EP - 568

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 2

ER -