EpCAM expression in the prostate cancer makes the difference in the response to growth factors

Go J. Yoshida, Hideyuki Saya

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Introduction: Epithelial cell adhesion molecule (EpCAM) is expressed in tumors with an epithelial cell of origin, in a heterogeneous manner. Prostate cancer stem-like cells highly express EpCAM. However, little is known about how EpCAM is involved in the ability of cells to adapt to micro-environmental changes in available growth factors, which is one of the essential biological phenotypes of cancer stem-like cells (CSCs). Methods: EpCAM-high and EpCAM-low subpopulations of cells were established from the prostate cancer cell line PC-3. Signal transductions in response to serum starvation, and on the exposure to EGF ligand or the specific inhibitor were analyzed in terms. Furthermore, we analyzed the expression level of amino acid transporters which contribute to the activation of mTOR signal between the two subgroups. Results: EpCAM-high and EpCAM-low PC-3 subpopulations showed markedly different responses to serum starvation. EpCAM expression was positively correlated with activation of the mTOR and epithelial growth factor receptor (EGFR) signaling pathways. Furthermore, AMP-activated protein kinase (AMPK) was gradually de-activated in EpCAM-low PC-3 cells in the absence of serum. Conclusions: EpCAM regulates the AMPK signaling pathway, essential for the response to growth factors characterized by EGF. LAT1, the amino acid transporter stabilized at the cellular membrane by EpCAM, is likely to be responsible for the difference in the susceptibility to EGF between EpCAM-high and EpCAM-low PC-3 cells.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume443
Issue number1
DOIs
Publication statusPublished - 2014 Jan 3

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Cell Adhesion Molecules
Prostatic Neoplasms
Intercellular Signaling Peptides and Proteins
Epidermal Growth Factor
Amino Acid Transport Systems
AMP-Activated Protein Kinases
Neoplastic Stem Cells
Starvation
Epithelial Cell Adhesion Molecule
Epithelial Cells
Serum
Chemical activation
Cells
Growth Factor Receptors
Signal transduction
Signal Transduction
Tumors

Keywords

  • Cancer stem-like cell
  • EGFR signaling
  • EpCAM
  • Heterogeneity
  • MTOR signaling
  • Prostate cancer

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

EpCAM expression in the prostate cancer makes the difference in the response to growth factors. / Yoshida, Go J.; Saya, Hideyuki.

In: Biochemical and Biophysical Research Communications, Vol. 443, No. 1, 03.01.2014, p. 239-245.

Research output: Contribution to journalArticle

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