Epidural analgesia prevents endotoxin-induced gut mucosal injury in rabbits

Shizuko Kosugi, Hiroshi Morisaki, Tomoyuki Satoh, Kimiaki Ai, Michiko Yamamoto, Junko Soejima, Ryohei Serita, Yoshifumi Kotake, Akitoshi Ishizaka, Junzo Takeda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the present study, we evaluated the effect of epidural analgesia on the alterations of gut barrier function elicited by endotoxin in rabbits. After the placement of an epidural catheter, 28 male rabbits were randomized into either 0.5% lidocaine (group E) or saline (group C) group. The solutions (0.4 mL/kg) were epidurally injected, followed by continuous infusion (0.1 mL · kg-1 · h-1) throughout the study period. Under a continuous infusion of lipopolysaccharide (15 μg · kg-1 · h-1), mean arterial blood pressure, intramucosal pH, and plasma thrombomodulin concentrations were measured. At 4 h, mean arterial blood pressure was lower (P < 0.05), intramucosal pH was higher (P < 0.01), and the progression of hemodilution more profound (P < 0.05) in group E versus group C, whereas plasma thrombomodulin levels were increased to a similar extent between the groups. With less wet-to-dry weight ratio of ileum, histopathological injury scores of gut mucosa were significantly less in group E versus group C(P < 0.01). In a separate series of experiments (n = 10 each group), mucosal permeability in group E was significantly less compared with group C (P < 0.05). Collectively, these studies showed that despite a significant decrease of perfusion pressure and arterial oxygen content, epidural analgesia minimized endotoxin-induced functional and structural injury of gut mucosa possibly through endothelium-independent mechanisms.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalAnesthesia and Analgesia
Volume101
Issue number1
DOIs
Publication statusPublished - 2005 Jul

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Epidural Analgesia
Endotoxins
Arterial Pressure
Rabbits
Wounds and Injuries
Thrombomodulin
Mucous Membrane
Hemodilution
Lidocaine
Ileum
Endothelium
Lipopolysaccharides
Permeability
Catheters
Perfusion
Oxygen
Weights and Measures

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Epidural analgesia prevents endotoxin-induced gut mucosal injury in rabbits. / Kosugi, Shizuko; Morisaki, Hiroshi; Satoh, Tomoyuki; Ai, Kimiaki; Yamamoto, Michiko; Soejima, Junko; Serita, Ryohei; Kotake, Yoshifumi; Ishizaka, Akitoshi; Takeda, Junzo.

In: Anesthesia and Analgesia, Vol. 101, No. 1, 07.2005, p. 265-272.

Research output: Contribution to journalArticle

Kosugi, S, Morisaki, H, Satoh, T, Ai, K, Yamamoto, M, Soejima, J, Serita, R, Kotake, Y, Ishizaka, A & Takeda, J 2005, 'Epidural analgesia prevents endotoxin-induced gut mucosal injury in rabbits', Anesthesia and Analgesia, vol. 101, no. 1, pp. 265-272. https://doi.org/10.1213/01.ANE.0000153863.95598.08
Kosugi, Shizuko ; Morisaki, Hiroshi ; Satoh, Tomoyuki ; Ai, Kimiaki ; Yamamoto, Michiko ; Soejima, Junko ; Serita, Ryohei ; Kotake, Yoshifumi ; Ishizaka, Akitoshi ; Takeda, Junzo. / Epidural analgesia prevents endotoxin-induced gut mucosal injury in rabbits. In: Anesthesia and Analgesia. 2005 ; Vol. 101, No. 1. pp. 265-272.
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