Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells

Yuki Saito, Akira Kunitomi, Tomohisa Seki, Shugo Tohyama, Dai Kusumoto, Makoto Takei, Shin Kashimura, Hisayuki Hashimoto, Gakuto Yozu, Chikaaki Motoda, Masaya Shimojima, Toru Egashira, Mayumi Oda, Keiichi Fukuda, Shinsuke Yuasa

Research output: Contribution to journalLetter

Abstract

The expression of pluripotency genes fluctuates in a population of embryonic stem (ES) cells and the fluctuations in the expression of some pluripotency genes correlate. However, no correlation in the fluctuation of Pou5f1, Zfp42, and Nanog expression was observed in ES cells. Correlation between Pou5f1 and Zfp42 fluctuations was demonstrated in ES cells containing a knockout in the NuRD component Mbd3. ES cells containing a triple knockout in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b showed correlation between the fluctuation of Pou5f1, Zfp42, and Nanog gene expression. We suggest that an epigenetic barrier is key to preventing the propagation of fluctuating pluripotency gene expression in ES cells.

Original languageEnglish
Pages (from-to)2879-2889
Number of pages11
JournalFEBS Letters
Volume591
Issue number18
DOIs
Publication statusPublished - 2017 Sep 1

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Keywords

  • embryonic stem cell
  • epigenome
  • fluctuation

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Saito, Y., Kunitomi, A., Seki, T., Tohyama, S., Kusumoto, D., Takei, M., Kashimura, S., Hashimoto, H., Yozu, G., Motoda, C., Shimojima, M., Egashira, T., Oda, M., Fukuda, K., & Yuasa, S. (2017). Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells. FEBS Letters, 591(18), 2879-2889. https://doi.org/10.1002/1873-3468.12791