Epinephrine augments von Willebrand factor-dependent shear-induced platelet aggregation

S. Goto, Y. Ikeda, M. Murata, M. Handa, E. Takahashi, A. Yoshioka, Y. Fujimura, M. Fukuyama, S. Handa, S. Ogawa

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Background. Shear-induced platelet aggregation (SIPA) is an important mechanism in thrombogenesis. von Willebrand factor (vWF) binding to platelet glycoprotein Ib (GP Ib) has been found to be crucial for platelet aggregation under the high shear force probably generated in stenosed coronary artery. The physiological significance of vWF-dependent SIPA has not been clarified. Methods and Results. Blood samples were collected from 23 normal volunteers. SIPA was continuously monitored using a modified cone-plate viscometer adapted for measuring the transmitted light intensity of the material. The effects of low concentrations of epinephrine, ADP, and collagen on SIPA under both low shear (12 dyne/cm2) and high shear (108 dyne/cm2) force were investigated. All agonists tested enhanced SIPA under low shear force, whereas only epinephrine augmented SIPA under high shear force. The maximum extents of SIPA under high shear force in the absence and presence of epinephrine (10 ng/ml) were 37.9±11.5% and 59.7±13.9%, respectively. The antagonist of the α2-adrenergic receptor yohimbine (1 μg/ml) antagonized the effects of epinephrine. The monoclonal antibody NMC-4 against vWF, which was shown to inhibit its binding to GP Ib, completely abolished SIPA under high shear force, even in the presence of epinephrine. However, this antibody only partially inhibited SIPA under low shear force. Conclusions. Our findings suggest that epinephrine is the agonist that enhances SIPA mediated by vWF through its specific receptor. This may be clinically important because occlusion of the coronary artery often occurs in stenosed atherosclerotic vessels under sympathetic stimulation.

Original languageEnglish
Pages (from-to)1859-1863
Number of pages5
JournalCirculation
Volume86
Issue number6
DOIs
Publication statusPublished - 1992 Jan 1

Keywords

  • epinephrine
  • platelets
  • von Willebrand factor
  • α-receptor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Epinephrine augments von Willebrand factor-dependent shear-induced platelet aggregation'. Together they form a unique fingerprint.

  • Cite this

    Goto, S., Ikeda, Y., Murata, M., Handa, M., Takahashi, E., Yoshioka, A., Fujimura, Y., Fukuyama, M., Handa, S., & Ogawa, S. (1992). Epinephrine augments von Willebrand factor-dependent shear-induced platelet aggregation. Circulation, 86(6), 1859-1863. https://doi.org/10.1161/01.CIR.86.6.1859