Epithelial overexpression of interleukin-32α in inflammatory bowel disease

M. Shioya, A. Nishida, Y. Yagi, A. Ogawa, T. Tsujikawa, S. Kim-Mitsuyama, A. Takayanagi, N. Shimizu, Y. Fujiyama, A. Andoh

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Interleukin (IL)-32 is a recently described proinflammatory cytokine, characterized by induction of nuclear factor (NF)-κB activation. We studied IL-32α expression in the inflamed mucosa of inflammatory bowel disease (IBD). We also investigated mechanisms regulating IL-32α expression. Tissue samples were obtained endoscopically or surgically from patients with ulcerative colitis (UC) (n = 10), Crohn's disease (CD) (n = 10), ischaemic colitis (n = 4) and normal colorectal tissues (n = 10). IL-32α expression was evaluated by standard immunohistochemical procedure. IL-32 mRNA expression was analysed by Northern blot. IL-32α was expressed weakly by colonic epithelial cells from normal individuals and subjects with ischaemic colitis. In the inflamed mucosa of IBD patients, epithelial IL-32α expression was increased markedly. In UC and CD patients, IL-32α expression was enhanced in affected mucosa compared to non-affected mucosa. In intestinal epithelial cell lines, expression of IL-32α mRNA and protein was enhanced by IL-1β, interferon (IFN)-γ and tumour necrosis factor (TNF)-α. A combination of TNF-α plus IFN-γ exerted synergistic effects. IL-32α induction by IL-1β and/or TNF-α was mediated by NF-κB activation. Epithelial IL-32α expression was increased in IBD patients, and in CD patients in particular. IL-32α might be involved in the pathophysiology of IBD as a proinflammatory cytokine and a mediator of innate immune response.

Original languageEnglish
Pages (from-to)480-486
Number of pages7
JournalClinical and Experimental Immunology
Volume149
Issue number3
DOIs
Publication statusPublished - 2007 Sep

Fingerprint

Interleukins
Inflammatory Bowel Diseases
Mucous Membrane
Crohn Disease
Ischemic Colitis
Tumor Necrosis Factor-alpha
Interleukin-1
Ulcerative Colitis
Interferons
Epithelial Cells
Cytokines
Messenger RNA
Innate Immunity
Northern Blotting

Keywords

  • Cytokine
  • IBD
  • NOD2

ASJC Scopus subject areas

  • Immunology

Cite this

Shioya, M., Nishida, A., Yagi, Y., Ogawa, A., Tsujikawa, T., Kim-Mitsuyama, S., ... Andoh, A. (2007). Epithelial overexpression of interleukin-32α in inflammatory bowel disease. Clinical and Experimental Immunology, 149(3), 480-486. https://doi.org/10.1111/j.1365-2249.2007.03439.x

Epithelial overexpression of interleukin-32α in inflammatory bowel disease. / Shioya, M.; Nishida, A.; Yagi, Y.; Ogawa, A.; Tsujikawa, T.; Kim-Mitsuyama, S.; Takayanagi, A.; Shimizu, N.; Fujiyama, Y.; Andoh, A.

In: Clinical and Experimental Immunology, Vol. 149, No. 3, 09.2007, p. 480-486.

Research output: Contribution to journalArticle

Shioya, M, Nishida, A, Yagi, Y, Ogawa, A, Tsujikawa, T, Kim-Mitsuyama, S, Takayanagi, A, Shimizu, N, Fujiyama, Y & Andoh, A 2007, 'Epithelial overexpression of interleukin-32α in inflammatory bowel disease', Clinical and Experimental Immunology, vol. 149, no. 3, pp. 480-486. https://doi.org/10.1111/j.1365-2249.2007.03439.x
Shioya, M. ; Nishida, A. ; Yagi, Y. ; Ogawa, A. ; Tsujikawa, T. ; Kim-Mitsuyama, S. ; Takayanagi, A. ; Shimizu, N. ; Fujiyama, Y. ; Andoh, A. / Epithelial overexpression of interleukin-32α in inflammatory bowel disease. In: Clinical and Experimental Immunology. 2007 ; Vol. 149, No. 3. pp. 480-486.
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