Epolactaene binds human Hsp60 Cys442 resulting in the inhibition of chaperone activity

Yoko Nagumo, Hideaki Kakeya, Mitsuru Shoji, Yujiro Hayashi, Naoshi Dohmae, Hiroyuki Osada

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Epolactaene is a microbial metabolite isolated from Penicillium sp., from which we synthesized its derivative ETB (epolactaene tertiary butyl ester). In the present paper, we report on the identification of the binding proteins of epolactaene/ETB, and the results of our investigation into its inhibitory mechanism. Using biotin-labelled derivatives of epolactaene/ETB, human Hsp (heat-shock protein) 60 was identified as a binding protein of epolactaene/ETB in vitro as well as in situ. In addition, we found that Hsp60 pre-incubated with epolactaene/ETB lost its chaperone activity. The in vitro binding study showed that biotin-conjugated epolactaene/ETB covalently binds to Hsp60. In order to investigate the binding site, binding experiments with alanine mutants of Hsp60 cysteine residues were conducted. As a result, it was suggested that Cys 442 is responsible for the covalent binding with biotin-conjugated epolactaene/ETB. Furthermore, the replacement of Hsp60 Cys442 with an alanine residue renders the chaperone activity resistant to ETB inhibition, while the alanine replacement of other cysteine residues do not. These results indicate that this cysteine residue is alkylated by ETB, leading to Hsp60 inactivation.

Original languageEnglish
Pages (from-to)835-840
Number of pages6
JournalBiochemical Journal
Volume387
Issue number3
DOIs
Publication statusPublished - 2005 May 1
Externally publishedYes

Fingerprint

Esters
Biotin
Alanine
Cysteine
epolactaene
Carrier Proteins
Chaperonin 60
Derivatives
Penicillium
Metabolites
Binding Sites
Experiments

Keywords

  • Chaperone activity
  • Citrate synthase
  • Covalent binding
  • Epolactaene
  • Heat-shock protein 60 (Hsp60)
  • Malate dehydrogenase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nagumo, Y., Kakeya, H., Shoji, M., Hayashi, Y., Dohmae, N., & Osada, H. (2005). Epolactaene binds human Hsp60 Cys442 resulting in the inhibition of chaperone activity. Biochemical Journal, 387(3), 835-840. https://doi.org/10.1042/BJ20041355

Epolactaene binds human Hsp60 Cys442 resulting in the inhibition of chaperone activity. / Nagumo, Yoko; Kakeya, Hideaki; Shoji, Mitsuru; Hayashi, Yujiro; Dohmae, Naoshi; Osada, Hiroyuki.

In: Biochemical Journal, Vol. 387, No. 3, 01.05.2005, p. 835-840.

Research output: Contribution to journalArticle

Nagumo, Y, Kakeya, H, Shoji, M, Hayashi, Y, Dohmae, N & Osada, H 2005, 'Epolactaene binds human Hsp60 Cys442 resulting in the inhibition of chaperone activity', Biochemical Journal, vol. 387, no. 3, pp. 835-840. https://doi.org/10.1042/BJ20041355
Nagumo, Yoko ; Kakeya, Hideaki ; Shoji, Mitsuru ; Hayashi, Yujiro ; Dohmae, Naoshi ; Osada, Hiroyuki. / Epolactaene binds human Hsp60 Cys442 resulting in the inhibition of chaperone activity. In: Biochemical Journal. 2005 ; Vol. 387, No. 3. pp. 835-840.
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