Epoxyquinol B, a naturally occurring pentaketide dimer, inhibits NF-κB signaling by crosslinking TAK1

Hiroshi Kamiyama, Takeo Usui, Hiroaki Sakurai, Mitsuru Shoji, Yujiro Hayashi, Hideaki Kakeya, Hiroyuki Osada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Several epoxyquinoids interfere with NF-κB signaling by targeting IKKβ or NF-κB. We report that epoxyquinol B (EPQB), classified as an epoxyquiniod, inhibits NF-κB signaling through inhibition of the TAK1 complex, a factor upstream of IKKβ and NF-κB. cDNA microarray analysis revealed that EPQB decreased TNF-α-induced expression of NF-κB target genes. EPQB covalently bound to a recombinant TAK1-TAB1 fusion protein in vitro, and inhibited its kinase activity. Furthermore, in vitro/in situ treatment with EPQB resulted in a ladder-like hypershift of TAK1 protein bands. We reported recently that EPQB crosslinks proteins via cysteine residues by opening its two epoxides, and our current results suggest that EPQB inhibits NF-κB signaling by crosslinking TAK1 itself or TAK1 through other proteins.

Original languageEnglish
Pages (from-to)1894-1900
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number7
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

IKappaB kinase
crosslinking
Dimers
Crosslinking
Proteins
proteins
ladders
Ladders
Microarrays
epoxides
cysteine
phosphotransferases (kinases)
Fusion reactions
Genes
Epoxy Compounds
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Cysteine
epoxyquinol B
Phosphotransferases

Keywords

  • Crosslink
  • Epoxyquinol B
  • NF-κB
  • TAK1

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Epoxyquinol B, a naturally occurring pentaketide dimer, inhibits NF-κB signaling by crosslinking TAK1. / Kamiyama, Hiroshi; Usui, Takeo; Sakurai, Hiroaki; Shoji, Mitsuru; Hayashi, Yujiro; Kakeya, Hideaki; Osada, Hiroyuki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 7, 2008, p. 1894-1900.

Research output: Contribution to journalArticle

Kamiyama, H, Usui, T, Sakurai, H, Shoji, M, Hayashi, Y, Kakeya, H & Osada, H 2008, 'Epoxyquinol B, a naturally occurring pentaketide dimer, inhibits NF-κB signaling by crosslinking TAK1', Bioscience, Biotechnology and Biochemistry, vol. 72, no. 7, pp. 1894-1900. https://doi.org/10.1271/bbb.80142
Kamiyama, Hiroshi ; Usui, Takeo ; Sakurai, Hiroaki ; Shoji, Mitsuru ; Hayashi, Yujiro ; Kakeya, Hideaki ; Osada, Hiroyuki. / Epoxyquinol B, a naturally occurring pentaketide dimer, inhibits NF-κB signaling by crosslinking TAK1. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 7. pp. 1894-1900.
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